基于纳米电极的单细胞内生物分子电化学分析OA北大核心CSTPCD
Electrochemical Analysis of Intracellular Molecules at Single Cells Based on Nanoelectrodes
单细胞分析能够更加精准地获取生物学信息,避免因平均化分析而丢失单细胞异质性特征,这对于研究阐明细胞代谢和信号通路至关重要.基于纳米电极的电化学分析技术因其高选择性、高灵敏度和高时空分辨率的优点而被广泛用于单细胞分析.本文综合评述了利用纳米电极对单细胞内部生物分子进行定量分析的最新研究进展,介绍了其在生物学研究中的应用,并对该领域面临的问题和未来发展进行了总结与展望.
Single-cell analysis enables more accurate acquisition of biological information and avoids the loss of single-cell heterogeneity features from averaging analysis,which is essential for studies to elucidate cell metabolism and signaling pathways.Electrochemical analysis techniques based on nanoelectrodes are widely used in single-cell analysis because of their high selectivity,high sensitivity and high spatiotemporal resolution.In this review,the latest research progress of the quantitative analysis of intracellular biomolecules in single cells using nanoelectrodes is reviewed.Moreover,its application in biological research and the challenges are introduced.
刘康;潘荣容;江德臣
南京大学化学化工学院,生命分析化学国家重点实验室,南京 210092
化学
单细胞单细胞器生物分子电化学分析纳米电极
Single cellSingle organelleBiomoleculeElectrochemistryNanoelectrode
《高等学校化学学报》 2024 (005)
1-9 / 9
国家杰出青年科学基金(批准号:22025403)资助. Supported by the National Science Fund for Distinguished Young Scholars,China(No.22025403).
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