化工进展2024,Vol.43Issue(4):2201-2209,9.DOI:10.16085/j.issn.1000-6613.2023-0541
单级固定床生物膜反应器主流厌氧氨氧化快速启动性能
Rapid start-up performance of mainstream Anammox in a single-stage fixed-bed biofilm reactor
摘要
Abstract
Mainstream anaerobic ammonium oxidation(Anammox)is a hotspot and difficulty issue for nitrogen removal from municipal wastewater.In this study,a single-stage fixed-bed biofilm reactor(SFBR)was used to treat simulated municipal wastewater.By switching from intermittent operation and controlling low-oxygen(DO:0.4-0.7mg/L),mainstream Anammox was successfully started up after 65 days,and the system achieved removal efficiencies of ammonia nitrogen and total nitrogen up to 98.8%and 92.6%,respectively.At this moment,the biofilm structure was dense and appeared red.Microbial activity test showed that the activity of Anammox bacteria(SAA)was the highest among all the functional bacteria.High-throughput sequencing confirmed that the co-existence of Anammox genus(Candidatus Brocadia),partial denitrification genus(Thauera)and nitrifying genus(Nitrospira),but ammonia oxidizing bacteria was not detected.Further analysis of the amoA functional gene amplicon indicated that Nitrospira was actually complete ammonia oxidizing(Comammox)bacteria.Therefore,the system achieved nitrogen removal from municipal wastewater through the coupled pathway of complete nitrification/partial denitrification/Anammox within an oxygen-stratified biofilm.These study findings provided a new process idea for the rapid realization of mainstream Anammox.关键词
生物膜/固定床/反应器/主流厌氧氨氧化/快速启动/脱氮Key words
biofilm/fixed-bed/reactor/mainstream Anammox/rapid start-up/nitrogen removal分类
资源环境引用本文复制引用
海岩,周鑫,李艳..单级固定床生物膜反应器主流厌氧氨氧化快速启动性能[J].化工进展,2024,43(4):2201-2209,9.基金项目
国家自然科学基金(21607111) (21607111)
山西省基础研究计划(20210302123198). (20210302123198)