河南农业科学2024，Vol.53Issue(4)：111-118,8.DOI:10.15933/j.cnki.1004-3268.2024.04.012
欧洲葡萄NF-YB3基因克隆与表达分析
Cloning and Expression Analysis of NF-YB3 Gene from Vitis vinifera
摘要
Abstract
The full length of the VvNF-YB3 gene was cloned from the European grape'Thompson Seedless'by PCR,to explore the function of the VvNF-YB3 gene in grape growth and development and response to abiotic stresses.The coding region sequence(CDS)of the VvNF-YB3 gene was 624 bp in length,encoding 207 amino acids,with an isoelectric point of 6.31,an instability index of 27.66,and a total average hydrophobic index of-0.738.Therefore,VvNF-YB3 was an acidic and stable hydrophilic protein.Through bioinformatics analysis,it was found that the VvNF-YB3 gene was located on chromosome 10 of the European grape,containing an intron and a CBFD_NFYB_HMF conserved domain,and showed the highest identity with VrNF-YB3 from Vitis riparia.The prediction result of secondary structure showed that VvNF-YB3 was mainly composed of α-helix and random coil.Correspondingly,the tertiary structure was a monomeric helix-coil-helix.The analysis of the cis-acting elements of the VvNF-YB3 gene promoter suggested that it might respond to hormones and stresses.To prove this inference,its expression patterns under different treatments were analyzed.The VvNF-YB3 gene was expressed in leaves,tendrils,flowers,young fruits,and stems of Red Globe,and the expression level was the highest in mature leaves.PEG simulated drought,low-temperature,and high-temperature stresses could change the expression level of the VvNF-YB3 gene,and VvNF-YB3 was a stress responsive gene.In addition,most of the interacting proteins of VvNF-YB3 were subunits of the nuclear factor Y family,and there were 27 transcription factors such as ERF,bHLH,ARF,etc.in the upstream of the VvNF-YB3 gene.It is speculated that the VvNF-YB3 gene may respond to various environmental stresses by being regulated by upstream transcription factors and interacting with other NF-Y proteins.关键词
葡萄/NF-YB3/基因克隆/生物信息学分析/表达分析Key words
Grape/NF-YB3/Gene cloning/Bioinformatics analysis/Expression analysis分类
农业科技引用本文复制引用
崔小月,尚泓泉,吕中伟,娄玉穗,张柯,樊红杰,吴文莹,张晓锋..欧洲葡萄NF-YB3基因克隆与表达分析[J].河南农业科学,2024,53(4):111-118,8.基金项目
国家葡萄产业技术体系豫东综合试验站项目(CARS-29-19) （CARS-29-19）
河南省科技攻关项目(222102110472) （222102110472）
河南省农业科学院优秀青年科技基金项目(2022YQ08) （2022YQ08）
河南省中央引导地方科技发展资金项目(Z20231811173) （Z20231811173）
