血清LRG1、NGAL和PGC-1α水平与小儿肾积水手术后分肾功能的相关性研究OA北大核心CSTPCD

Objective To explore the correlation between serum levels of leucine rich alpha-2-glycopro-tein 1(LRG1),neutrophil gelatinase associated apolipoprotein(NGAL),peroxisome proliferator activated re-ceptor γ coactivator factor-1α(PGC-1α)and postoperative differential renal fundion(DRF)in children with hydronephrosis.Methods From March 2019 to June 2022,124 children with hydronephrosis undergoing pye-loureteroplasty were selected as study subjects.Based upon postoperative DRF at Month 18,they were assigned into two groups of DRF ≥45％(n=72)and DRF＜45％(n=52).Enzyme-linked immunosorbent assay(ELISA)was applied for detecting the serum levels of LRG1,NGAL and PGC-1α.Pearson's correlation was u-tilized for examining the correlation between serum levels of LRG1,NGAL,PGC-1α and renal function.Multiva-riate Logistic regression was employed for analyzing the factors affecting postoperative DRF＜45％.Receiver op-erating characteristic(ROC)curve was used for evaluateing the serum values of LRG1,NGAL and PGC-1α in postoperative DRF in children with hydronephrosis.Results As compared with DRF ≥45％group,DRF＜45％group had higher levels of serum LRG1,NGAL,serum creatinine(Scr),blood urea nitrogen(BUN),andβ2-microglobulin(β2-MG)and lower levels of PGC-1α and preoperative DRF at affected side(P＜0.05).Ac-cording to Pearson's correlation analysis,LRG1 and NGAL were correlated positively with Scr,BUN and β2-MG(P＜0.05).And PGC-1 α was correlated negatively with β2-MG,Scr and BUN(P＜0.05).Multivariate Logis-tic regression analysis indicated that serum LRG1,NG AL,preoperative DRF at affected side,Scr,BUN and P2-MG were risk factors affecting postoperative DRF＜45％while PGC-1α was a protective factor affecting postop-erative DRF＜45％(P＜0.05).According to ROC curve analysis,area under the curve(AUC)of serum LRG1,NGAL,PGC-1α and their combined evaluation for postoperative DRF＜45％was 0.899,0.872,0.878 and 0.982 respectively.And the combination of three factors was superior to each alone(Z three combined e-valuation-LRG1=3.148,Z three combined evaluation-NGAL=3.937,Z three combined evaluation-PGC-1α=3.125,P＜0.05).Conclusions There are the elevations of serum levels of LRG1 and NGAL and a decline of PGC-lα in hydronephrosis children with postoperative DRF＜45％.The combined detection of the three has some evaluation values for postoperative DRF..