热带作物学报2024，Vol.45Issue(4)：674-681,8.DOI:10.3969/j.issn.1000-2561.2024.04.003
五指毛桃不同部位补骨脂素合成酶基因的表达及其代谢产物含量
Expression of Psoralen Synthase Gene and Effect of Its Product Syn-thesis in Different Parts of Ficus hirta Vahl
摘要
Abstract
To explore the relationship between the expression of psoralen synthase gene(PS)and psoralen synthesis,quantitative real-time PCR(qPCR)and high performance liquid chromatography(HPLC)were used to determine the expression of PS and the content of psoralen in roots,stems,leaves and fruits of Ficus hirta Vahl(FH)from Guangxi and Fujian.PS was expressed in roots,stems,leaves and fruits of Guangxi and Fujian FH,and the expression was tis-sue-specific.The relative expression level in roots was the highest(expression in branch roots was higher than that in principal roots),followed by leaves(expression in entire margined leaves were higher than that in incised leaves),and the expression levels in stems and fruits were extremely low.The psoralen contents of FH had some differences from the two producing areas.They were accumulated in the principal root,branch root,entire margined leaf,incised leaf,stem and fruit from Guangxi and Fujian provinc FH,and the contents were the highest in branch roots,which was 3.206 mg/g and 2.947 mg/g respectively,followed by principal root(2.136 mg/g and 0.419 mg/g).The content in incised leaves was 0.022 mg/g and 0.028 mg/g respectively,that in entire margined leaves was 0.069 mg/g and 0.035 mg/g.The psoralen content of stem(0.003 mg/g and 0.003 mg/g)and fruit(0.002 mg/g and 0.001 mg/g)was lower than other parts.The psoralen content was correlated with the expression of PS.The study would provide insights into understanding the role of PS in the psoralen metabolic in Ficus hirta Vahl.关键词
五指毛桃/补骨脂素/补骨脂素合成酶/基因表达/工艺优化/含量Key words
Ficus hirta Vahl/psoralen/psoralen synthetase/gene expression/process optimization/content分类
农业科技引用本文复制引用
杨琳,杨帆,张杭颖,黄勃诚,邱清华,张君诚..五指毛桃不同部位补骨脂素合成酶基因的表达及其代谢产物含量[J].热带作物学报,2024,45(4):674-681,8.基金项目
福建省科技厅面上项目(No.2020J01382) （No.2020J01382）
福建省教育厅中青年教师教育科研项目(No.JAT220359). （No.JAT220359）
