热带作物学报2024，Vol.45Issue(4)：682-690,9.DOI:10.3969/j.issn.1000-2561.2024.04.004
辣椒种质资源PMMoV抗性基因检测与抗性鉴定
Resistance Gene Detection and Resistance Identification of PMMoV in Pepper Germplasm
摘要
Abstract
Pepper mild mottle virus(PMMoV)is one of the most serious pathogens worldwide.Currently,five patho-genic types of PMMoV have been reported,including P0,P1,P1,2,P1,2,3 and P1,2,3,4.The wide host range and strong pathogenicity of PMMoV seriously affect the yield and quality of pepper,and breeding for disease resistance is the most economical and effective means to control this virus.Our previous study determined that PMMoV infected with Dan-zhou pepper germplasm nursery in Hainan is the P1,2 pathogenic type.In order to screen PMMoV-resistant pepper germplasm resources,110 germplasms were tested for PMMoV resistance using the reported L3-linked molecular mark-ers.46 pepper germplasms containing molecular markers of resistance were inoculated with PMMoV P1,2 pathogenic type by inoculation at seeding stage.Local symptoms were investigated 5 d after inoculation,and systemic symptoms were investigated 20 d after inoculation,and disease indices were calculated.The results showed that there were sig-nificant differences in disease resistance among different pepper germplasms,and the disease indexes of various germ-plasms ranged from 8.86-60.00.The germplasms showing high resistance(HR)to PMMoV P1,2 pathogenic type were CF19-34m and 19FB6-1.The germplasms showing resistance(R)wasCCJ93-1.The germplasms showing me-dium-resistance(MR)included 17YB32,17SCa28m,15SM39-2,14SM555×14SM1,14SM567-2,14SM526-1,14SM565-1,14SM502-1,14SM519-1,14SM504m,14SM503m,14SM519-3,14SM518-2,14SM519-2,CCJ52-1,etc.The presenting susceptible(S)germplasms were 14SM514m,CCJ22-1,CCJ113-2,CCJ135-1,CCJ157-1,L537-1m,L545,CCJ87-2 and CCJ133-1,and the obtained disease-resistant germplasms accounted for 80.43%of the total identi-fied materials.The results of this study would lay the foundation for creating germplasm resources resistant to pepper mild mottle virus and breeding resistant varieties.关键词
辣椒/种质资源/PMMoV/分子标记/抗病性鉴定Key words
pepper(Capsicum annuum L.)/germplasm/PMMoV/molecular markers/disease resistance identification分类
农业科技引用本文复制引用
陈建分,曹振木,秦于玲,申龙斌,刘维侠,朱丹,吴怡婷,刘子记,王旭..辣椒种质资源PMMoV抗性基因检测与抗性鉴定[J].热带作物学报,2024,45(4):682-690,9.基金项目
海南省自然科学基金面上项目(No.322MS132) （No.322MS132）
中央级公益性科研院所基本科研业务费专项(No.1630032022009). （No.1630032022009）
