热带作物学报2024，Vol.45Issue(4)：703-711,9.DOI:10.3969/j.issn.1000-2561.2024.04.006
89份香蕉种质资源SRAP分子标记亲缘关系分析
Genetic Relationship Analysis among 89 Banana Germplasms Based on SRAP Molecular Markers
摘要
Abstract
Banana classification is a difficult problem,using morphological features to classify is unable to meet the needs.Molecular marker technology has been gradually applied to population identification and classification,genetic relationship analysis and genetic diversity research of banana varieties(lines).In this paper,SRAP molecular marker technology was used to analyze the genetic relationship of 89 banana germplasm resources.Using 10 pairs of positive and negative primers to form 100 pairs of primer combinations,and 13 pairs of SRAP primers with easy identification,clear band type and high polymorphism were screened out.170 identifiable bands were amplified by the 13 pairs of primers,with an average of 13.08 bands per pair of primers,of which 140 bands showed polymorphism,with a poly-morphism ratio of 79.00%.The similarity coefficient of 89 banana germplasm resources varied from 0.241 to 1.000,and the genetic diversity was very rich.The similarity coefficient of the same type of wild banana from the same place or different places was high.When the similarity coefficient was 0.49,the 89 banana resources could be divided into six groups.The first major category were AAA group Xiangyajiao,AA group Gongjiao,AAB group Longyajiao and AA group wild banana(AAA,AA,AAB).The second major category were ABB group Fenjiao,ABB group Fendajiao and BB group wild banana(ABB,BB).The third major category were Dajiao and a banana wild relative Musa itinerans.M coccinea,Musella lasiocarpa,Heliconia belonged respectively to the fourth,fifth and sixth category.The results are basically consistent with the current classical classification conclusion.Only some banana germplasm have different classification status,indicating that it is feasible to use SRAP markers to classify banana germplasm resources.At the same time,the cultivated Xiangya banana,Gongya banana and Longya banana have the same ancestry,and are close to the wild banana of AA genotype,while the wild banana of BB genotype is close to the pink banana,the pink banana and the wild banana of BB genotype,and the banana germplasm resources of different genomes are gathered together,which showing that the origin of banana germplasm resources affects the genetic relationship of offspring,and a single classi-fication method cannot completely distinguish the banana germplasm resources.In addition,the genetic relationship between plantain and plantain is relatively close,but it is impossible to distinguish whether plantain contains A and B genomes,so the genotype and genetic background of plantain need further study.关键词
香蕉/SRAP/聚类分析/亲缘关系Key words
banana/SRAP/cluster analysis/genetic relationship分类
农业科技引用本文复制引用
周海琪,刘丽琴,夏玲,吕顺,曾莉莎,王芳,黄晓彦,陈东仪,刘文清,梁少丽..89份香蕉种质资源SRAP分子标记亲缘关系分析[J].热带作物学报,2024,45(4):703-711,9.基金项目
东莞市2021年度省乡村振兴战略专项资金"大专项+任务清单"(No.20211800400052) （No.20211800400052）
广东省级农业科技创新及推广项目"香蕉菠萝产业技术体系创新团队"(No.2023KJ109) （No.2023KJ109）
2022年东莞市科技特派员项目(No.20221800500062). （No.20221800500062）
