热带作物学报2024，Vol.45Issue(4)：742-751,10.DOI:10.3969/j.issn.1000-2561.2024.04.010
低温胁迫下不同甘蔗品种的生理响应及耐寒性评价
Physiological Response and Cold Tolerance Evaluation of Different Sugarcane Varieties under Low Temperature Stress
摘要
Abstract
Low temperature is one of the main environmental factors affecting the growth,yield and distribution of sug-arcane.It is of great significance to explore the physiological response characteristics of sugarcane under low tempera-ture stress and screen strong cold-tolerant sugarcane parent materials for guiding sugarcane breeding and promoting the development of sugarcane industry.In this study,eight different sugarcane varieties and one sugarcane wild species(Erianthus fulvus 99-1 clone)were used as the experimental materials to study the physiological index response after low temperature stress treatment,and the cold tolerance of the nine materials was comprehensively evaluated by prin-cipal component analysis,fuzzy membership function method and cluster analysis.The contents of malondialdehyde(MDA),chlorophyll(Chl),soluble sugar(SS)and soluble protein(SP)in most materials were higher than those of CK after low temperature stress(3℃,3 days).The content of proline(Pro)in all materials was higher than that of CK, while the activity of superoxide dismutase(SOD)was lower.The activities of peroxidase(POD)and catalase(CAT)in most materials were higher than those of CK.According to the comprehensive evaluation value(D)and cluster analysis,the nine materials were divided into three groups:strong cold resistance type(E.fulvus 99-1,Yacheng 89-9,ROC10),moderate cold resistance type(ROC20,Yuetang 93-159,Huangjiali,ROC16)and weak cold resistance type(Dianzhe 01-58,ROC22).The results indicate that different sugarcane varieties have different adaptability to low temperature.Strong cold-tolerant sugarcane varieties can quickly adjust themselves under adversity,reasonably increase the content of osmotic adjustment substances and the activity of antioxidant enzymes,further remove harmful substances such as reactive oxygen species(ROS)in the body,and balance cell metabolism process,so as to better adapt to low temperature environment.This study would lay a foundation for exploring the regulation mechanism of sugarcane response to low temperature stress and breeding new sugarcane varieties with strong cold tolerance.关键词
甘蔗/耐寒性/生理响应/主成分分析/聚类分析/综合评价Key words
sugarcane/cold resistance/physiological response/principal component analysis/cluster analysis/compre-hensive evaluation分类
农业科技引用本文复制引用
饶席兵,钱禛锋,曾丹,张蓉琼,吕绍芝,沈庆庆,吴华英,何丽莲,李富生..低温胁迫下不同甘蔗品种的生理响应及耐寒性评价[J].热带作物学报,2024,45(4):742-751,10.基金项目
云南省重大科技专项(No.202202AE090021) （No.202202AE090021）
云南省作物生产与智慧农业重点实验室专项(No.202105AG070007) （No.202105AG070007）
国家自然科学基金项目(No.31960451). （No.31960451）
