热带作物学报2024，Vol.45Issue(4)：837-846,10.DOI:10.3969/j.issn.1000-2561.2024.04.019
基因沉默番木瓜环斑病毒复制酶基因(PRSV-Nib)获得抗病毒病番木瓜的研究
Gene Silencing of Papaya ringspot virus Replicase Gene(PRSV-Nib)to Obtain Virus Resistant Papaya
摘要
Abstract
Papaya is an economically important tropical fruit.Papaya ringspot virus(PRSV)is an important virus dis-ease of papaya,often causing significant yield losses and quality deterioration.Since the introduction of the first trans-genic papaya in 1998,PDR-based breeding strategies for disease resistance have been successfully applied.The contra-diction between disease resistance based on sequence homology and increased virus genetic diversity became a new challenge for papaya breeding.In this study,we propose to use RNAi strategies aim at nuclear inclusion b gene(Nib)to obtain broad-spectrum resistance to PRSV papaya.With optimized embryo callus generation-Agrobatium meidated transformation-shoot regeneration,52 shoots were obtained after resistance screening and a total of 24 transgenic posi-tive shoots were obtained by specific PCR screening for the T0 generation.In the T0 generation field natural disease test,the transgenic papaya strains were significantly more resistant to disease than the non-transgenic control,with NibB5-2 having the best resistance in the field.Molecular charaterization of the insertion site of Nib5-2 was located at position 1976766 on chromosome 2 supercontig_30 by hiTAIL-PCR method.Green house inoculation test of NibB5-2 T1 gen-eration,there was no virus accumulation and no disease symptoms,confirmed the resistance.Our resulst reveal that RNAi silcencing the Nib gene enhance the papaya virus resistance,meanwhile enriched the candidated genes for virus resistance breeding.关键词
番木瓜/番木瓜环斑病毒/Nib基因/RNA介导的病毒抗性Key words
Carica papaya L./Papaya ringspot virus/Nib gene/RNA-mediated virus resistance分类
农业科技引用本文复制引用
吴清铧,贾瑞宗,郭静远,杨牧之,胡玉娟,郝志刚,赵辉,郭安平..基因沉默番木瓜环斑病毒复制酶基因(PRSV-Nib)获得抗病毒病番木瓜的研究[J].热带作物学报,2024,45(4):837-846,10.基金项目
海南省重大科技计划项目(No.ZDKJ202002) （No.ZDKJ202002）
海南省重点研发计划项目(No.ZDYF2022XDNY257) （No.ZDYF2022XDNY257）
崖州湾科技城菁英人才项目(No.SCKJ-JYRC-2022-67). （No.SCKJ-JYRC-2022-67）
