水产学报2024，Vol.48Issue(4)：136-146,11.DOI:10.11964/jfc.20220413421
长期碳酸盐碱度胁迫对脊尾白虾生长及卵巢发育的影响
Effects of long-term saline-alkaline stress on growth and ovary development of Exopalaemon carinicauda
摘要
Abstract
In order to investigate the effects of carbonate alkalinity(CA)on the growth and ovary development of the ridgetail white prawn Exopalaemon carinicauda,three CA gradients of 3 mmol/L(control group),5 mmol/L and 8 mmol/L were set to culture E.carinicauda for 60 days,and the effects of different CA stress on the growth performance,tissue structure,enzyme activity and ovary development were compared.The results showed that there was no significant difference in growth gain,weight gain or survival rate between 5 mmol/L and control groups(P>0.05),while those of 8 mmol/L group was significantly lower than control group(P<0.05).Hepatopan-creas sections showed that the morphological structure of the lumen was severely deformed in 8 mmol/L group.Gill sections showed that gill filaments were swollen,blood cells swelled,epithelial cells were seriously damaged,and the stratum corneum space was deformed in 8 mmol/L group.Carbonic anhydrase activity in 5 mmol/L group was significantly higher than control group(P<0.05),while Na+/K+-ATPase activity in muscle showed no signific-ant difference(P>0.05);there was no significant difference in carbonic anhydrase in hepatopancreas(P>0.05),and Na+/K+-ATPase activity in hepatopancreas of 8 mmol/l group was significantly lower than other groups(P<0.05).Na+/K+-ATPase activity in gill of two stress groups was significantly higher than control group(P<0.05).Ovaries of all three groups were able to develop,while only 3 mmol/l and 5 mmol/L groups had E.carinicauda ovary developed to stage Ⅲ;ovary sections showed that the arrangement of oocytes in 8 mmol/L group was loose,and the arrangement of surrounding follicular cells was loose and the number was small.To sum up,E.carinicauda can grow and develop in CA less than 8 mmol/L,but high CA may cause damage to the gills and hepatopancreas,thus affecting growth.At the same time,it is speculated that high CA may also affect the development rate of ovar-ies.This study provides basic data and reference information for the enhancement and cultivation of white shrimps in saline-alkaline water.关键词
脊尾白虾/碳酸盐碱度胁迫/生长/组织切片/酶活性/卵巢发育Key words
Exopalaemon carinicauda/carbonate alkalinity stress/growth/tissue sections/enzyme activity/ovary development分类
农业科技引用本文复制引用
张秀红,李吉涛,王佳佳,王成伟,秦桢,葛倩倩,刘萍,李健..长期碳酸盐碱度胁迫对脊尾白虾生长及卵巢发育的影响[J].水产学报,2024,48(4):136-146,11.基金项目
国家重点研发计划(2018YFD0901302) （2018YFD0901302）
国家自然科学基金(32072974) （32072974）
现代农业产业技术体系专项(CARS-48) （CARS-48）
中国水产科学研究院基本科研业务费(2023TD50) National Key R&D Program of China(2018YFD0901302) （2023TD50）
National Natural Science Founda-tion of China(32072974) （32072974）
China Agriculture Research System of MOF and MARA(CARS-48) （CARS-48）
Central Public-interest Scientific Institution Basal Research Fund,CAFS(2023TD50) （2023TD50）
