水产学报2024，Vol.48Issue(4)：159-170,12.DOI:10.11964/jfc.20220313357
夏季北黄海贝类养殖区环境因子、长牡蛎糖原和免疫指标变化的调查
Investigation on the variation of environmental factors,glycogen and immune parameters of Pacific oyster(Crassostrea gigas)in the North Yellow Sea shellfish farming area in summer
摘要
Abstract
Summer mass mortality is one of the major bottlenecks limiting the green and high-quality develop-ment of the Pacific oyster Crassostrea gigas aquaculture in China.The frequent environmental stresses in summer,including high-temperature,rainfall,pathogen stimulation,picophytoplankton abundance and reproductive beha-vior,are important causes of mass mortality.Monitoring the changes of key environmental factors and health-related indicators in the aquaculture of C.gigas will help to forecast the risk of mass mortality.In this study,two surveys were conducted for C.gigas aquaculture in the North Yellow Sea during the summer heat and rainfall period to monitor the variations of water parameters,picophytoplankton abundance,bacterial abundance,glyco-gen content and immune-related indicators.It was found that,for the first and second survey in July,the surface water temperature was 25.3 and 24.9 ℃,respectively.The salinity for the two surveys was 26.41 and 27.87,respectively.The abundance of diatoms for the two surveys was 3.6×103 and 3×103 cells/L,respectively,which was lower than the average level of previous reports,and the diversity of dinoflagellates increased.For the two sur-veys,the abundance of total bacteria in the water was 2.10×109 and 2.77×1010 copies/L,respectively,and the abundance of Vibrio was 3.37×108 and 5.40×108 copies/L,respectively.The glycogen content of hepatopancreas decreased to 40.96 and 31.58 mg/g respectively for the two surveys,and the glycogen content of adductor muscle decreased to 6.63 and 8.91 mg/g,respectively.MDA content in gill was 13.74 and 8.69 nmol/mg prot for the two surveys,respectively,and T-AOC in gill was 0.46 and 0.77 U/mg prot,respectively.The expression levels of CgIL17-5 and CgTNF-1 mRNA in haemocytes dramatically increased in the first survey compared to the oysters cultured indoor.The expression levels of CgCaspase3 mRNA in haemocytes increased compared to the oysters cultured indoor,but the differences were not significant.In conclusion,the results showed higher water temperat-ure and lower salinity happened in shellfish farming areas in the summer of 2021.The abundance of diatoms decreased compared to the average level of previous reports,and the diversity of dinoflagellates,a potentially harmful algae,increased.The abundance of environmental bacteria changed during the environmental stresses,and the risk of outbreak of potential pathogenic bacteria Vibrio increased.Organism responses were observed in oysters including lower glycogen content and mild oxidative stress.These results help us to further understand the environmental patterns and response characteristics of the organism during summer and provided a theoretical basis and reference for the prevention and control of the mass oyster mortality in summer.关键词
长牡蛎/高温/降雨/糖原/免疫/北黄海Key words
Crassostrea gigas/high temperature/rainfall/glycogen/immune/North Yellow Sea分类
农业科技引用本文复制引用
高磊,王文彪,李磊,王玲玲,宋林生,孔宁,刘冉阳,赵俊彦,邢镇,张子杨,赵宝,李庆嵩,付强..夏季北黄海贝类养殖区环境因子、长牡蛎糖原和免疫指标变化的调查[J].水产学报,2024,48(4):159-170,12.基金项目
国家重点研发计划(2018YFD0900606) （2018YFD0900606）
国家自然科学基金国际(地区)合作与交流项目(41961124009) （地区）
农业农村部:国家现代农业产业技术体系(CARS-49) （CARS-49）
大连市科技创新基金(2021JJ13SN73) （2021JJ13SN73）
辽宁省应用基础研究计划(2022JH2/101300140) National Key Research and Development Program of China(2018YFD0900606) （2022JH2/101300140）
Funds for International Cooperation and Exchange of the National Natural Science Foundation of China(41961124009) （41961124009）
China Agriculture Research System of MOF and MARA(CARS-49) （CARS-49）
Dalian Science and Technology Innovation Fund Project(2021JJ13SN73) （2021JJ13SN73）
Liaoning Applied Basic Research Program(2022JH2/101300140) （2022JH2/101300140）
