水产学报2024，Vol.48Issue(4)：255-266,12.DOI:10.11964/jfc.20230313957
脑室注射葡萄糖对翘嘴鳜摄食和糖代谢的影响
Effects of intracerebroventricular injection of glucose on feeding and glucose metabolism in mandarin fish(Siniperca chuatsi)
摘要
Abstract
Diets containing high levels of carbohydrate could inhibit the feeding in many fish species,but the rel-evant mechanisms behind this regulation are not clear.The mandarin fish(Siniperca chuatsi),a typical carnivor-ous fish,exhibits an apparent anorexia due to its extensive intolerance to dietary carbohydrates,which limits its value as a cultured species.To investigate the existence of a glucose sensing system in the hypothalamus of S.chuatsi and its effect on feed intake,intracerebroventricular(ICV)injections of saline(control),2-DG(glucose metabolism antagonist,100 mg/kg)and glucose(10 mg/kg)were performed,exploring the impacts on feeding and glucose metabolism at 3,6 and 12 h.The results showed that ICV of glucose significantly inhibited the feed intake of mandarin fish,while the plasma glucose levels remained constant at different time points.The expression of glucokinase(gk)were significantly induced at 6 h after ICV administration of glucose,indicating the presence of a glucose-sensing system in the hypothalamus of mandarin fish.ICV administration of glucose significantly pro-moted the mRNA levels of the cocaine and amphetamine-regulated transcriptional peptide(cart)at 3 h,which may be related to the significant upregulation of mTOR in the hypothalamus(12 h)and liver(6 h)of mandarin fish.In addition,ICV administration of glucose significantly induced the expression of gk(6 h)and pk(3 h)in the liver of mandarin fish to promote glycolysis,suggesting the promotion of catabolism for energy supply through per-ceived glucose levels in the hypothalamus.In conclusion,this study demonstrates for the first time that gk in the hypothalamus can response to the glucose level,suggesting the potential existence of glucose sensing system in the hypothalamus of mandarin fish,and that ICV administration of glucose regulates the AMPK/mTOR pathway through gk-induced glucose sensing,which in turn regulates the expression of appetite-related genes to inhibit feed intake.The present study would provide a theoretical basis for research on the carbohydrate utilization and feeding regulation in mandarin fish.关键词
翘嘴鳜/葡萄糖感知/脑室注射/摄食调控/糖代谢Key words
Siniperca chuatsi/glucose-sensing system/intracerebroventricular injection/feeding regulation/gluc-ose metabolism分类
农业科技引用本文复制引用
曾妍芝,郁二蒙,李红燕,王广军,谢骏,田晶晶,龚望宝,夏耘,张凯,李志斐..脑室注射葡萄糖对翘嘴鳜摄食和糖代谢的影响[J].水产学报,2024,48(4):255-266,12.基金项目
广东省基础与应用基础研究基金(2023A1515010008) （2023A1515010008）
中国水产科学研究院基本科研业务费专项(2022SJ-XK1) （2022SJ-XK1）
国家现代农业产业技术体系建设专项(CARS-45-21) Guangdong Basic and Applied Basic Research Foundation(2023A1515010008) （CARS-45-21）
Central Pub-lic-interest Scientific Institution Basal Research Fund,CAFS(2022SJ-XK1) （2022SJ-XK1）
China Agriculture Research System(CARS-45-21) （CARS-45-21）
