水产学报2024，Vol.48Issue(4)：267-279,13.DOI:10.11964/jfc.20231214291
牛磺酸缓解脂质氧化日粮对黄河鲤生长性能和肠道健康的负面影响
Taurine alleviated the negative effects of lipid oxidation diet on growth performance and intestinal health of Yellow River carp(Cyprinus carpio)
摘要
Abstract
Lipid oxidation is prevalent in fish feed,which reduces the nutritional value of fish feed and damages the health of aquatic animals.Taurine has been extensively studied as a feed additive to promote fish growth and antioxidant capacity,but its effects on intestinal health need further research.To investigate the effects of taurine on the growth performance and intestinal health of the Yellow River carp(Cyprinus carpio)fed with a lipid-oxid-ized diet,a total of 225 C.carpio with similar weight at about 8.74 g were randomly divided into five groups:FO,OFO,T0.4,T0.8,and T1.2,respectively.A 10-week feeding trial was performed.The results showed that taurine supplementation alleviated the growth inhibition caused by the OFO diet on C.carpio,mainly by improving final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and feed efficiency(FE).The oxidized lipid diet inhibited nrf2 mRNA expression in the hepatopancreas and intestine and promoted the mRNA levels of keap1 in the intestine.Appropriate taurine addition can inhibit the decrease of nrf2 mRNA levels and increase of keap1 mRNA levels induced by the OFO diet.Taurine also increased mRNA levels of antioxidation-related genes(gr,gpx and sod).For example,supplementation of 0.8 g/kg taurine significantly increased intestinal gr,gpx,and sod expression levels.In addition,taurine mitigated the decreased intestinal digestive enzyme activity(lipase,amylase,and trypsin),villus height,villus width,and muscular thickness of the oxidized lipid diet.Moreover,taur-ine restored the decreased alpha-diversity index(Chao1,observed species,Shannon,and Simpson index)of intest-inal flora induced by the oxidized lipid diet.Furthermore,supplementation of 0.8%taurine reversed intestinal flora disturbance caused by the oxidized lipid diet,mainly by reducing the abundance of pathogenic bacteria(Aeromonasd and Acinetobacter,etc.)and increasing the abundance of beneficial bacteria(Lactobacillus,Cetobac-terium,and Prevotella,etc.).In conclusion,taurine mitigated the negative effects of lipid oxidized diet on the growth performance and intestinal health of C.carpio,and the recommended supplemental level of taurine in the oxidized lipid diet was 0.4%-0.8%.This study lays a theoretical foundation for further exploring the biological function of taurine in fish intestines.关键词
黄河鲤/牛磺酸/脂质氧化/生长性能/肠道健康Key words
Yellow River carp(Cyprinus carpio)/taurine/lipid oxidation/growth performance/intestinal health分类
农业科技引用本文复制引用
刘丹,米佳丽,王俊丽,闫潇,秦超彬,杨丽萍,徐歆歆,聂国兴..牛磺酸缓解脂质氧化日粮对黄河鲤生长性能和肠道健康的负面影响[J].水产学报,2024,48(4):267-279,13.基金项目
国家自然科学基金(32072991,U22A20532) （32072991,U22A20532）
河南省现代农业产业技术体系建设专项(HARS-22-16-G2) （HARS-22-16-G2）
河南师范大学创新科技队伍建设项目(2020TD02) National Natural Science Foundation of China(32072991,U22A20532) （2020TD02）
Special Fund for Henan Agriculture Research System(HARS-22-16-G2) （HARS-22-16-G2）
Innovation Scientists and Technicians Troop Construc-tion Projects of Henan Normal University(2020TD02) （2020TD02）
