水产学报2024，Vol.48Issue(4)：336-345,10.DOI:10.11964/jfc.20221213853
大黄鱼UBXN1基因鉴定及其过表达后的转录组分析
Identification of UBXN1 and transcriptome analysis after its overexpression in large yellow croaker(Larimichthys crocea)
摘要
Abstract
Scuticociliatia disease is a major threat to large yellow croaker Larimichthys crocea at the fry stage.In the previous study,a ubiquitin regulatory X domain-containing protein(UBXN1)gene was found to be involved in Scuticociliatia disease resistance through genome-wide association analysis.To further confirm its gene function in resistance against Scuticociliatia,we identified UBXN1 gene sequence and investigated its expression characterist-ics,as well as signal pathway.The complete cDNA sequence of UBXN1 was 915 bp,encoding 304 amino acids.The putative UBXN1 protein was evolutionarily conserved and contained a UBA and a UBX domain.Moreover,UBXN1 had varying degrees of expressions in different tissues,among which the highest expression was observed in brain,moderate expression was in liver,heart and kidney,and the lowest expression was in muscle by qRT-PCR detection.It also showed that induced expression was found in spleen,brain,liver,and kidney during the early phase of infection,and the expression gradually recovered to the control level at the late phase.Further subcellular localization analysis showed that UBXN1 uniformly distributed in cytoplasm and nucleus.In addition,16 differen-tially expressed genes were found in a comparative transcriptome analysis between the UBXN1 over expression group and control vector group,including up-regulated genes RPL41/RPL39/XIST/RNA45SN4 and down-regulated genes ATP8/ND4L.These findings provide new insights into finfish UBXN1 and its potential regulatory signaling pathway.关键词
大黄鱼/包含泛素调节性X结构域的蛋白(UBXN1)/盾纤毛虫/转录组/免疫Key words
Larimichthys crocea/ubiquitin regulatory X domain-containing protein,UBXN1/Scuticociliaia/tran-scriptome/immune分类
生物科学引用本文复制引用
张东玲,唐欣,王志勇..大黄鱼UBXN1基因鉴定及其过表达后的转录组分析[J].水产学报,2024,48(4):336-345,10.基金项目
国家自然科学基金(32172964) （32172964）
福建省自然科学基金重点项目(2022J02042) National Natural Science Foundation of China(32172964) （2022J02042）
Key Projects of Fujian Provincial Natural Science Foundation(2022J02042) （2022J02042）
