药学研究2024，Vol.43Issue(4)：333-337,5.DOI:10.13506/j.cnki.jpr.2024.04.004
黄芩苷对SARS-COV-2侵袭的抑制作用研究
Inhibitory effect of baicalin on SARS-COV-2 invasion
摘要
Abstract
Objective To study the antiviral efficacy of baicalin on SARS-COV-2 entry process in vitro.Methods The SARS-CoV-2 S pseudo-typed virions containing a luciferase reporter gene and Luciferase kit were used to detect the changes of fluorescein expression caused by baicalin in Huh-7 cells after incubation with the pseudo-typed virion,and then the virus inhibition curve is plotted.Results Baicalin can significantly inhibited the infection rate of pseudo virus.There was no signifi-cant difference in the virus inhibition curve between the baicalin&virus pre-incubation group and co-incubation at different concentrations,indicating that baicalin could not directly bind to virus,but could play an antiviral role by affecting the cell fu-sion process mediated by the virus S protein.We further found that,the inhibition rate of baicalin to virus decreased signifi-cantly in the 4 h group,but had no significant difference in the 0 h and 2 h groups at the concentration of 0.125 mg·mL-1,in-dicating that baicalin may have an inhibitory effect on virus invasion stage rather than adsorption stage,and the mediated inhi-bition stage occurred within 4 h.Conclusion Baicalin may mediate the fusion of SARS-CoV-2 S protein with cell surface re-ceptor and exert anti-SARS-CoV-2 activity by playing a role in inhibiting virus invasion in non-adsorption stage.关键词
黄芩苷/新型冠状病毒/荧光素酶检测/SARS-CoV-2S蛋白Key words
Baicalin/SARS-CoV-2/Luciferase assay/SARS-CoV-2 S protein分类
中医学引用本文复制引用
耿雪,王雪,周倩,苏昕宇,李水仙,祝清芬..黄芩苷对SARS-COV-2侵袭的抑制作用研究[J].药学研究,2024,43(4):333-337,5.基金项目
山东省自然科学基金重点项目(No.ZR2020KH021) （No.ZR2020KH021）
山东省食品药品检验研究院院级课题(No.SDIFDC-KY-2021009) （No.SDIFDC-KY-2021009）
