陕西医学杂志2024，Vol.53Issue(5)：579-582,588,5.DOI:10.3969/j.issn.1000-7377.2024.05.001
信号转导和转录激活因子3/CC趋化因子配体2信号通路对乳腺癌细胞活力、迁移和侵袭的影响实验研究
Effect of signal transducer and activator of transcription 3/C-C motif chemokine ligand 2 signaling pathway on viability,migration and invasion of breast cancer cells
摘要
Abstract
Objective:To investigate the effect of signal transducer and activator of transcription 3(STAT 3)/C-C motif chemokine ligand 2(CCL2)signaling pathway on viability,migration and invasion of breast cancer cells.Methods:Human breast cancer cell lines(HCC1937,MCF-7,MDA-MB-231,ZR-71-1)and human normal breast epithelial cells(MCF-10A)were cultured in vitro.The expressions of p-STAT3,STAT3 and CCL2 in HCC1937,MCF-7,MDA-MB-231,ZR-75-1 and MCF-10A cells were detected by Western blot.Human breast cancer cells with the highest p-STAT3/STAT3 protein ratio and CCL2 protein expression were selected for follow-up experiments.MCF-7 cells were divided into control group,STAT3 inhibitor(WP1066)group Ⅰ(2 μmol/L WP1066),group Ⅱ(4 μmol/L WP1066)and groupⅢ(8 μmol/L WP1066).Cell count kit 8,scratch test and Transwell test were used to detect MCF-7 cell viability,mobility and invasion ability,respectively.The protein expressions of p-STAT3,STAT3,CCL2,matrix metalloprotei-nase(MMP)-2 and MMP-9 in MCF-7 cells were detected by Western blot.Results:Compared with MCF-10A cells,the expressions of p-STAT3/STAT3 protein ratio and CCL2 protein were increased in HCC1937,MCF-7,MDA-MB-231 and ZR-75-1 cells(all P＜0.05).Among them,the p-STAT3/STAT3 protein ratio and CCL2 protein expression were the highest in MCF-7 cells,so MCF-7 cells were selected for follow-up experiments.Compared with the control group,MCF-7 cells viability,mobility and invasion number in STAT3 inhibitor groups Ⅰ,Ⅱ and Ⅲ were decreased successively(all P＜0.05).Compared with the control group,the p-STAT3/STAT3 protein ratio,CCL2,MMP-2 and MMP-9 protein expressions of MCF-7 cells in STAT3 inhibitor groups Ⅰ,Ⅱ and Ⅲ were decreased successively(all P＜0.05).Conclusion:STAT3/CCL2 is highly expressed in breast cancer cells,and inhibition of STAT3/CCL2 signaling pathway can reduce the viability,migration and invasion of breast cancer cells.关键词
乳腺癌/信号转导和转录激活因子3/CC趋化因子配体2/细胞活力/细胞迁移/细胞侵袭Key words
Breast cancer/Signal transducer and activator of transcription 3/C-C motif chemokine ligand 2/Cell viability/Cell migration/Cell invasion分类
医药卫生引用本文复制引用
薛杰,张永梅,陈启,郝艳萍..信号转导和转录激活因子3/CC趋化因子配体2信号通路对乳腺癌细胞活力、迁移和侵袭的影响实验研究[J].陕西医学杂志,2024,53(5):579-582,588,5.基金项目
内蒙古自治区自然科学基金资助项目(2019MS08150) （2019MS08150）
