人毛囊真皮鞘细胞移植治疗兔跟腱炎实验研究OACSTPCD

Objective:To investigate the therapeutic effect of human hair follicle dermal sheath cells(hHF-DSCs)transplantation on achilles tendonitis of rabbit.Methods:The hHF-DSCs were isolated and cultured from the hair fol-licles of human posterior occipital.Thirty male white rabbits were randomly divided into control group,model group and treatment group,with 10 rabbits in each group.The model group and the treatment group were given multiple in-jection of type Ⅰ collagenase 1 cm above the left achilles tendon insertion point to establish the rabbit model of achil-les tendinitis.On day 0,3,6 and 13 after the successful establishment of the achilles tendinitis model,0.5 ml of hHF-DSCs were injected into the affected side of the achilles tendon in the treatment group,and the same volume of 0.9％sodium chloride solution was injected into the same part of the achilles tendon in the model group and the control group.The appearance and histopathological staining of achilles tendon in each group were observed.The con-tent of hydroxyproline(HYP)and the expression levels of tendinocyte C(TNC),matrix metalloproteinase-9(MMP-9),interleukin(IL)-1β,IL-6,IL-10 and tumor necrosis factor-α(TNF-α)were detected by ELISA.The expression levels of collagen Ⅰ and Ⅲ in achilles tendon tissues were detected by immunohistochemical method.The biomechanics of achilles tendon tissue was measured by stretch method.Results:CD14,CD34,CD45 and HLA-DR were almost not expressed on the surface of hHF-DSCs after isolation and culture,and the expression rates of CD49d,CD90,CD73 and CD105 were close to 100％.Compared with the control group,the gait of the model group was obviously changed,and the achilles tendon was red,swollen,inelastic and dull.The results of HE staining showed that the collagen fibers in the model group had loose arrangement,few prokaryotic cells and uneven distribution,indicating that the rabbit mod-els of achilles tendinitis were successfully constructed.The elasticity and gloss of the achilles tendon tissue in the treatment group were better than those in the model group,and HE staining showed that the collagen fibers in the treatment group were arranged neatly,no obvious fiber necrosis,fewer inflammatory cells,and normal number of col-lagen fiber prokaryotic cells.HYP content in model group was lower than that in control group and treatment group,while that in control group was higher than that in treatment group(all P＜0.05).The level of MMP-9 in model group was higher than that in control group and treatment group,and that in treatment group was higher than that in control group(all P＜0.05).The TNC level of model group was lower than that of control group and treatment group,and that of treatment group was lower than that of control group(all P＜0.05).Compared with the control group,the expression levels of IL-1β,IL-6,IL-10 and TNF-α in the achilles tendon tissues of rabbits in the treatment group and model group were increased(all P＜0.05).Compared with model group,the expression levels of IL-1β,IL-6 and TNF-α in treatment group were decreased(all P＜0.05).Compared with model group,collagen Ⅰ and Ⅲin treatment group were increased(all P＜0.05).Compared with control group,collagen Ⅰ and Ⅲ in model group were decreased(all P＜0.05).The maximum load of achilles tendon in the model group was lower than that in the control group,and that in the treatment group was higher than that in the model group(all P＜0.05).Conclusion:hHF-DSCs transplantation in the treatment of rabbit achilles tendinitis can promote the repair of damaged achilles tendon tissue and collagen synthesis,reduce collagen degradation and alleviate inflammatory response.