他莫昔芬联合左炔诺孕酮对大鼠子宫肌瘤的影响及机制研究OACSTPCD

Objective:To investigate the effects of tamoxifen combined with levonorgestrel on uterine fibroids in rats and their mechanism.Methods:Thirty adult female SD rats with SPF grade were selected to establish uterine fi-broid model by intrabitoneal injection of estradiol benzoate combined with progesterone.The rats were randomly di-vided into control group(normal rats were intragastric administration with 2 ml 0.9％sodium chloride solution),model group(uterine fibroids rats were intragastric administration with 2 ml 0.9％sodium chloride solution),tamoxifen group(uterine fibroids rats were intragastric administration with 1.70 mg/kg tamoxifen)and levonorg-estrel group(uterine fibroids rats were intragastric administration with 0.1 mg/kg levonorgestrel)and combination group(tamoxifen combined with levonorgestrel intragastric administration in uterine fibroid rats),6 rats in each group were given continuous intragastric administration for 30 days.Serum levels of estradiol(E2),progesterone,fol-licle-stimulating hormone(FSH)and luteinizing hormone(LH)were detected by ELISA.Uterine coefficient,length of left and right cervical horn and maximum diameter of uterine horn were measured.The structure and endometrial thickness of uterus were observed by HE staining.The expressions of Wnt5b and β-catenin protein in uterine tissue were detected by Western blot.Results:Compared with the control group,the serum levels of E2,progesterone,FSH and LH in the model group were increased(all P＜0.05).The serum levels of E2,progesterone,FSH and LH in combination group were lower than those in tamoxifen group and levonorgestrel group(all P＜0.05).Uterine coeffi-cient,length of left and right cervical horn,maximum diameter of uterine horn and endometrial thickness in model group were increased compared with those in control group(all P＜0.05).Uterine coefficient,length of left and right cervical corners,maximum diameter of uterine horn and endometrial thickness in combination group were lower than those in tamoxifen and levonorgestrel groups(all P＜0.05).The degree of uterine injury in combination group was less than that in model group.The expressions of Wnt5b and β-catenin protein in uterine tissue of model group were higher than those of control group(all P＜0.05).The expressions of Wnt5b and β-catenin protein in combination group were lower than those in tamoxifen group and levonorgestrel group(all P＜0.05).Conclusion:Tamoxifen combined with levonorgestrel can shrink uterine fibroids,improve uterine morphology,and inhibit the growth of uter-ine smooth muscle cells in rats.The mechanism may be related to the inhibition of Wnt/β-catenin pathway.