陕西医学杂志2024，Vol.53Issue(5)：593-597,5.DOI:10.3969/j.issn.1000-7377.2024.05.004
苍术素调节RhoA/ROCK信号通路对弥漫大B细胞淋巴瘤细胞恶性生物学行为的影响
Effects of atractylodin on the malignant biological behavior of diffuse large B-cell lymphoma cells by regulating the RhoA/ROCK signaling pathway
摘要
Abstract
Objective:To explore the effects of atractylodin(ATR)on the malignant biological behavior of dif-fuse large B-cell lymphoma(DLBCL)cells by regulating the Ras homolog gene family member A(RhoA)/Rho as-sociated coiled-coil containing protein kinase(ROCK)signaling pathway.Methods:DLBCL cells SUDHL-4 were routinely cultured and randomly grouped into control group,low concentration ATR group(ATR-L group,5 μmol/L ATR),medium concentration ATR group(ATR-M group,10 μmol/L ATR),high concentration ATR group(ATR-H group,20 μmol/L ATR),and high concentration ATR+RhoA activator U46619 group(ATR+U46619 group,20 μmol/L ATR+10 nmol/L U46619).CCK-8 method was applied to measure cell proliferation activity.Wound healing test was applied to test the cell migration ability.Transwell experiment was applied to measure cell invasion ability.Flow cytometry was applied to detect cell apoptosis rate.Real-time fluorescence quantitative PCR(RT-qPCR)method was applied to determine the expressions of RhoA and ROCK1 mRNA in cells.Western blot was applied to determine the expressions of Bcl-2,Bax,RhoA,and ROCK1 proteins of cells in each group.Results:Compared with the control group,the OD450 value(48 h,72 h),cell migration rate,cell invasion number,RhoA,ROCK1 mRNA and protein expressions,and Bcl-2 protein expression of SUDHL-4 cells in ATR-M group and ATR-H group were de-creased,the apoptosis rate and Bax protein expression were increased(all P＜0.05).RhoA activator U46619 attenua-ted the inhibitory effect of ATR on the malignant biological behavior of DLBCL cells.Conclusion:ATR may inhibit the malignant biological behavior of DLBCL cells by down-regulating the RhoA/ROCK signaling pathway.关键词
弥漫大B细胞淋巴瘤/苍术素/RhoA/ROCK通路/细胞凋亡/细胞增殖/细胞侵袭/细胞迁移Key words
Diffuse large B-cell lymphoma/Atractylodin/RhoA/ROCK pathway/Apoptosis/Cell prolifera-tion/Cell invasion/Cell migration分类
医药卫生引用本文复制引用
张薇,陈姣敏,许卫星,尹凤雷,王娟..苍术素调节RhoA/ROCK信号通路对弥漫大B细胞淋巴瘤细胞恶性生物学行为的影响[J].陕西医学杂志,2024,53(5):593-597,5.基金项目
河北省中医药管理局科研计划项目(2023454) （2023454）
