甘草甜素对喉癌Hep-2细胞侵袭和迁移的影响及机制研究OACSTPCD

Objective:To explore the effect of glycyrrhizin(Gly)on invasion and migration of laryngeal carcinoma Hep-2 cells and its mechanism.Methods:Normal human laryngeal mucosal epithelial cells and laryngeal cancer Hep-2 cells were cultured in vitro,and the microRNA-205-5p(miR-205-5p),silent mating type information regulation 2 homolog-3(SIRT3)mRNA and protein levels were detected by real-time fluorescent quantitative PCR(RT-qPCR)and western blot assay.Laryngeal cancer Hep-2 cells were divided into control group,Gly low concentration group,Gly medium concentration group,Gly high concentration group,Gly high concentration+empty plasmid group,Gly high concentration+miR-205-5p in-hibitor group.Each group was given Gly intervention with corresponding concentration or transfected with corresponding plasmid for 48 h.The invasion and migration ability of Hep-2 cells in each group were detected by Transwell assay and scratch assay respectively.The miR-205-5p,SIRT3 mRNA and protein levels of Hep-2 cells in each group were detected by RT-qPCR and Western blot.The targeting relationship between miR-205-5p and SIRT3 was analyzed by double luciferase reporter gene assay.Results:Compared with human laryngeal mucosal epithelial cells,the levels of miR-205-5p in laryngeal cancer Hep-2 cells were decreased,and the levels of SIRT3 mRNA and protein were increased(all P＜0.05).Compared with control group,the number of transmembrane cells,scratch healing rate,SIRT3 mRNA and protein levels in Gly low,medium and high concentration groups were decreased successively,and the levels of miR-205-5p were increased successively(all P＜0.05).Compared with Gly high concentration group and Gly high concentration+empty plasmid group,the number of transmembrane cells,scratch healing rate,SIRT3 mRNA and protein levels in Gly high concentration+miR-205-5p inhibi-tor group were increased,and the level of miR-205-5p was decreased(all P＜0.05).miR-205-5p could target the regulation of SIRT3 expression.Conclusion:Gly can inhibit the invasion and migration of Hep-2 cells,and the mechanism may be relat-ed to the up-regulation of miR-205-5p expression and the targeted down-regulation of SIRT3 expression.