陕西医学杂志2024，Vol.53Issue(5)：610-615,6.DOI:10.3969/j.issn.1000-7377.2024.05.007
丹参酮ⅡA通过调控p38MAPK/NF-κB信号通路减轻糖尿病雄性大鼠生殖损伤实验研究
Tanshinone Ⅱ A alleviates reproductive damage in diabetic male rats by regulating p38MAPK/NF-κB signaling pathway
摘要
Abstract
Objective:To investigate the effect of tanshinone Ⅱ A(Tan Ⅱ A)on reproductive damage in diabetic male rats and its mechanism.Methods:Randomly selected thirty-five out of fourty-three SD male rats were used to prepare the diabetic animal model by feeding with high glucose and fat combined with intraperitoneal injection of streptozotocin,and which were divided into the model group,Tan Ⅱ A(6 mg/kg)group,SB203580(p38 mitogen ac-tivated protein kinase[p38MAPK]inhibitor,2 mg/kg)group and Tan Ⅱ A+SB203580(6 mg/kg+2 mg/kg)group,with eight rats in each group.And another eight rats were set as normal group.After four weeks of medica-tion,the level of fasting blood glucose(FBG)was detected.ELISA method was used to detect the content of serum testosterone(T),luteinizing hormone(LH)and follicle-stimulating hormone(FSH).The sperm quality(sperm con-centration,survival rate and vitality)was analyzed.The quality of testis was weighed and calculating testicular index.HE staining was used to observe the pathological changes of testis tissue.The activity of total superoxide dismutase(T-SOD),catalase(CAT)and the content of malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleu-kin-iβ(IL-1β),IL-8 in testicular tissue were determined.Western blot method was used to detect the expression of p38MAPK,p-p38MAPK,nuclear factor-KB p65(NF-κB p65),p-NF-κB p65,high mobility group protein B1(HMGB1).Results:Compared with the model group,there was no significant difference in FBG level of Tan Ⅱ A,SB203580 and TanⅡ A+SB203580 groups(all P＞0.05).The content of T,LH,FSH and the sperm concentration,survival rate,vitality were increased(all P＜0.05).The testis mass and testis index were increased(all P＜0.05).The pathological changes of testis tissue were improved.The activity of T-SOD,CAT and the content of MDA,TNF-α,IL-1β,IL-8 were decreased(all P＜0.05).The ratio of p-p38MAPK/p38MAPK,p-NF-KB p65/NF-κB p65 and the expression of HMGB1 were decreased(all P＜0.05).The effects of Tan Ⅱ A+SB203580 on the indexes were better than those of Tan Ⅱ A group and SB203580 group(all P＜0.05).Conclusion:Tan Ⅱ A may reduce reproductive dam-age in male diabetic rats by inhibiting p38MAPK/NF-κB signaling pathway,reducing oxidative stress and inflamma-tory response.关键词
糖尿病/生殖损伤/丹参酮Ⅱ A/p38丝裂原活化蛋白激酶/核因子-κB信号通路/氧化应激/炎症反应/大鼠Key words
Diabetes mellitus/Reproductive damage/Tanshinone Ⅱ A/p38MAPK/NF-κB signaling pathway/Oxidative stress/Inflammatory response/Rats分类
医药卫生引用本文复制引用
杨芳,高素文,刘旋,韦志坤,郑聪聪..丹参酮ⅡA通过调控p38MAPK/NF-κB信号通路减轻糖尿病雄性大鼠生殖损伤实验研究[J].陕西医学杂志,2024,53(5):610-615,6.基金项目
河北省医学科学研究课题(20210509) （20210509）
