陕西医学杂志2024，Vol.53Issue(5)：684-687,691,5.DOI:10.3969/j.issn.1000-7377.2024.05.024
乳腺癌患者血清微小RNA-135b、微小RNA-148a-3p、微小RNA-186-5p表达水平及其与新辅助化疗效果相关性研究
Levels of serum miR-135b,miR-148a-3p and miR-186-5p in breast cancer patients and their correlation with the effect of neoadjuvant chemotherapy
摘要
Abstract
Objective:To investigate the expression levels of serum microRNA-135b(miR-135b),miR-148a-3p and miR-186-5p in breast cancer patients and their correlation with the effect of neoadjuvant chemotherapy.Methods:A total of 116 patients with breast cancer were selected as the case group,and all received neoadjuvant chemothera-py,21 days as a cycle,a total of 8 cycles of chemotherapy.According to the chemotherapy response,patients were di-vided into the drug-resistant group(32 cases)and the sensitive group(84 cases),and 128 healthy women were se-lected as the control group.The expression levels of serum miR-135b,miR-148a-3p and miR-186-5p were compared between the case group and the control group,and between the drug-resistance group and the sensitive group.Pearson correlation analysis was used to analyze the correlation between serum miR-135b,miR-148a-3p and miR-186-5p lev-els.Logistic regression analysis was used to analyze the influencing factors of chemotherapy effect in breast cancer patients.The predictive value of serum miR-135b,miR-148a-3p and miR-186-5p for chemotherapy resistance in breast cancer patients was analyzed by receiver operating characteristic(ROC)curve.Results:Compared with the control group,the levels of miR-135b and miR-148a-3p in the case group were increased,and the level of serum miR-186-5p was decreased(all P＜0.05).The levels of serum miR-135b and miR-148a-3p in the drug-resistant group were higher than those in the sensitive group,and the level of serum miR-186-5p was lower than that in the sensitive group(all P＜0.05).Pearson correlation analysis showed that serum miR-135b was positively correlated with miR-148a-3p and negatively correlated with miR-186-5p,and serum miR-148a-3p was negatively correlated with miR-186-5p(all P＜0.05).Multivariate Logistic regression analysis showed that serum miR-135b,miR-148a-3p and miR-186-5p were influencing factors of chemotherapy effect in breast cancer patients(all P＜0.05).ROC curve anal-ysis showed that the area under the curve(AUC)of the combination of serum miR-135b,miR-148a-3p and miR-186-5p for predicting chemotherapy resistance was higher than that of the three alone(all P＜0.05).Conclusion:The levels of serum miR-135b and miR-148a-3p in breast cancer patients are highly expressed,and the level of serum miR-186-5p is low.The changes of the three levels are related to the effect of neoadjuvant chemotherapy,and the combined detection of the three has a higher predictive value for neoadjuvant chemotherapy resistance in patients.关键词
乳腺癌/微小RNA-135b/微小RNA-148a-3p/微小RNA-186-5p/新辅助化疗/相关性研究Key words
Breast cancer/miR-135b/miR-148a-3p/miR-186-5p/Neoadjuvant chemotherapy/Correlation study分类
医药卫生引用本文复制引用
王玉龙,张美荣,周昱..乳腺癌患者血清微小RNA-135b、微小RNA-148a-3p、微小RNA-186-5p表达水平及其与新辅助化疗效果相关性研究[J].陕西医学杂志,2024,53(5):684-687,691,5.基金项目
黑龙江省卫生健康委员会科研项目(20230404080178) （20230404080178）
