实验科学与技术2024，Vol.22Issue(2)：8-13,6.DOI:10.12179/1672-4550.20230079
流式分选仪的创新调试及在初始T细胞纯化中的应用
Creative Adjustment of the Flow Cell Sorter and the Application in the Purification of Naive T Cells
摘要
Abstract
How to adjust the flow cell sorter for the sensitive cells is a concern of many practitioners.Using the FACSAria Fusion as an example,the article creatively combined the service interface with the user interface to adjust the lowest default sample pressure and then sorted the primary naive T cells according to the standard user's manual with the 85μm nozzle.The testing quality of the purified naive T cells revealed that the procedure could lead to high purity products.Moreover,treg cells differentiated from the products could generate Foxp3 normally compared with the non-sorting control.关键词
流式分选/进样压力/初始T细胞/调节T细胞/Foxp3Key words
flow cytometry sorting/sample pressure/naive T cell/treg cell/Foxp3分类
生物学引用本文复制引用
万雅娟,王瑞,张松,周颖,龙加福..流式分选仪的创新调试及在初始T细胞纯化中的应用[J].实验科学与技术,2024,22(2):8-13,6.基金项目
南开大学大型仪器实验技术研发项目(21NKSYJS07). （21NKSYJS07）
