实验科学与技术2024，Vol.22Issue(2)：47-53,7.DOI:10.12179/1672-4550.20230474
过氧化物酶的固定及同工酶鉴定综合实验设计
Comprehensive Experimental Design of Immobilization and Isoenzyme Identification of Peroxidase
摘要
Abstract
With strong theoretical and practical significance,the principles and applications of enzymology are a key and difficult content in the course of biochemistry.In order to improve students'comprehensive understanding and application ability of enzyme knowledge,a comprehensive experimental project"immobilization and isoenzyme identification of peroxidases"is designed.It integrates the main content of enzymes and focuses on five sections,namely,enzyme preparation,activity determination,characterization of enzymatic properties(Michaelis constant and stability),immobilization,and isoenzyme identification.Students'ability to analyze and solve problems are improved by deep participation and coordinated implementation,and the experiment achieves a good teaching effect.关键词
生物化学/综合性实验/酶/固定化/科研能力Key words
biochemistry/comprehensive experiment/enzyme/immobilization/scientific research ability分类
教育学引用本文复制引用
李晨,谭宗花,杨晓月,李娇..过氧化物酶的固定及同工酶鉴定综合实验设计[J].实验科学与技术,2024,22(2):47-53,7.基金项目
山西省高等学校教学改革创新项目(J20220065) （J20220065）
山西省自然科学基金(202203021221015) （202203021221015）
山西省研究生教育教学改革课题(2021YJJG002,2023JG018). （2021YJJG002,2023JG018）
