实验科学与技术2024，Vol.22Issue(2)：113-119,7.DOI:10.12179/1672-4550.20230284
藻毒素降解酶A与微囊藻毒素作用条件的分析
Study on the Interaction Conditions between Microcystinase A and Microcystins
摘要
Abstract
Targeting regulatory enzymes is an important aspect of chemical biology.The approach involves acquiring target enzymes through heterologous expression,studying their properties,and ultimately applying them in practical settings.Microcystinase A(MlrA)was identified and successfully prepared and characterized as a target enzyme.The study analyzed both the purity of the enzyme and its resulting effects,examining how different substrate concentrations,temperature,pH,and metal ions affected MlrA's activity in microcystins(MCs)degradation.Through experimentation and exploration,students can learn to prepare and analyze target proteins,design experiments to explore enzyme activity under different conditions,and use key instruments,which improves their problem-solving skills,their ability to apply chemical biology knowledge,and their scientific inquiry and social responsibility.In the process of experimental teaching,students were guided to think about the value and significance of scientific research to society,realize the importance of scientific ethics and social responsibility,and understand the social responsibility and mission of scientific researchers,which cunltivate their senses of scientific inquiry and social responsibility.关键词
化学生物学/研究型设计实验/藻毒素降解酶A/微囊藻毒素/酶活性Key words
chemical biology/research-oriented experimental design/microcystinase A/microcystins/enzyme activity分类
生物科学引用本文复制引用
郭小亮,殷月玲,吴彦东,冯玲玲,郑骧羊,徐国睿,周凡惠..藻毒素降解酶A与微囊藻毒素作用条件的分析[J].实验科学与技术,2024,22(2):113-119,7.基金项目
国家自然科学基金面上项目(22277037,21472061) （22277037,21472061）
中央高校基本科研业务费专项资金资助科研转化为教学项目(CCNU22KYZHSY05,CCNU16KYZHSY22) （CCNU22KYZHSY05,CCNU16KYZHSY22）
武汉知识创新专项基础研究项目(20220208-01010276). （20220208-01010276）
