实用医学杂志2024，Vol.40Issue(9)：1191-1196,6.DOI:10.3969/j.issn.1006-5725.2024.09.003
铜离子激活MAPK-ERK通路调控鼻咽癌放射敏感性
Copper regulates the radiosensitivity of nasopharyngeal carcinoma by activating MAPK-ERK signaling pathway
摘要
Abstract
Objective To Investigate the effect of copper ions on the radiosensitivity of nasopharyngeal carcinoma(NPC)and identify potential targets to improve the efficacy of NPC radiation therapy.Methods The content of copper ions in normal nasopharyngeal epithelial cell line NP69 and a variety of NPC cell lines was detected by a copper microplate assay.After adding 0,5,10,20,50,100,200 μmol/L copper ion solution and 0,0.05,0.1,0.2,0.5,1.0,2.0 mmol/L TEPA solution,CCK-8 assay was used to determine the effect of intracellular copper ion content on the survival rate of NPC cells before and after irradiation.And determine the drug concentration to be used in subsequent experiments.CCK-8 assay was used to determine the effect of intracellular copper ion content on the survival rate of NPC cells before and after irradiation,and to determine the drug concentration used in the copper ion group and TEPA group in the subsequent experiments.CCK-8 assay and clone formation assay were used to detect the changes in radiosensitivity of NPC cells after drug treatment.The DNA damage and MAPK-ERK signaling pathway-related protein expression of NPC cells in each group before and after irradiation were detected by Western blot.Results The content of copper ions in NPC cells was significantly higher than that in normal naso-pharyngeal epithelial cells(P＜0.05).When the concentration of copper ion solution was lower than 50 μmol/L,the radiation resistance of CNE1 cells was improved,and the radiation sensitivity of SUNE1 cells was increased by 0.1～0.2 mmol/L TEPA(P＜0.05).Compared with the control group,the NPC cell radiotherapy resistance of copper ion group was enhanced,and the NPC cell radiotherapy sensitivity of copper ion group was enhanced(P＜0.05).Compared with the control group,the activation degree of MAPK-ERK pathway in copper ion group was up-regulated(P＜0.05).MAPK-ERK pathway inhibitor SCH772984 effectively reversed copper-mediated NPC radiotherapy resistance(P＜0.05).Conclusion The increased copper ion content in NPC cells promoted the radiotherapy resistance of NPC cells by activating the MAPK-ERK signaling pathway.关键词
鼻咽癌/铜离子/MAPK-ERK通路/放射治疗Key words
nasopharyngeal carcinoma/copper ion/MAPK-ERK signaling pathway/radiation therapy分类
医药卫生引用本文复制引用
黄秀婷,林颉,叶晓心,蔡佳佐,袁亚维..铜离子激活MAPK-ERK通路调控鼻咽癌放射敏感性[J].实用医学杂志,2024,40(9):1191-1196,6.基金项目
国家自然科学基金项目(编号:82102974,82373516) （编号:82102974,82373516）
广州市科技计划项目(编号:202201011048) （编号:202201011048）
