实用医学杂志2024，Vol.40Issue(9)：1211-1217,7.DOI:10.3969/j.issn.1006-5725.2024.09.006
circRNA SIPA1L1修饰牙髓干细胞来源外泌体促血管生成能力的机制
Study on the angiogenic ability of exosomes derived from dental pulp stem cells modified by circRNA SIPA1L1
摘要
Abstract
Objective To investigate the effect of human pulp stem cells(hDPSC)-derived exosomes(Exo)modified by circRNA(circRNA)signal-induced proliferation-associated protein-like 1(SIPA1L1)on the angiogenesis of human umbilical vein endothelial cells(HUVEC).Methods hDPSC was isolated and cultured from pulp tissue,the circSIPA1L1 overexpression plasmid was transfected into hDPSC,and Exo was isolated and identified.HUVEC was divided into control group,hDPSC Exo group and circSIPA1L1-hDPSC Exo group.After culture for 48 h,the vasculoformation ability was detected by Matrigel matrix gel vasculoformation test,the expres-sion levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR2)and placental growth factor(PGF)were determined by qRT-PCR and Western blot.Results The Exo was success-fully isolated from the untransfected hDPSC and the hDPSC transfected with circSIPA1L1,and compared with the HDPSC-derived Exo,the relative expression of circSIPA1L1 in the hDPSC Exo transfected with circSIPA1L1 was significantly up-regulated(P＜0.05).Compared with hDPSC Exo group,the number of HUVEC tubular structures in circSIPA1L1-hDPSC Exo group was significantly increased(P＜0.05),and the mRNA and protein relative expressions of VEGF,VEGFR2 and PGF were also significantly up-regulated(P＜0.05).Conclusions Modification of hDPSC-derived Exo by circRNA SIPA1L1 can promote angiogenesis,and the mechanism may be related to up-regulation of VEGF,VEGFR2 and PGF expression levels.关键词
牙髓干细胞/环状RNA/信号诱导增殖相关蛋白1样蛋白1/外泌体/血管生成Key words
dental pulp stem cell/circRNA/signal-induced proliferation-associated protein-like protein-1/exosome/angiogenesis分类
医药卫生引用本文复制引用
刘景,冷春涛,王艳..circRNA SIPA1L1修饰牙髓干细胞来源外泌体促血管生成能力的机制[J].实用医学杂志,2024,40(9):1211-1217,7.基金项目
新疆维吾尔自治区自然科学基金项目(编号:2021D01C431) （编号:2021D01C431）
