摘要
Abstract
This study aims to develop an efficient and economical method to analyze the content of diethylstilbestrol in feed using high-performance liquid chromatography(HPLC)and photodiode detector(PDA).In order to avoid misjudgment at low concentrations,high-performance liquid chromatography mass spectrometry(HPLC/MS)was also used for re-confirmation.In the experiment,the feed samples were extracted with methanol.After shaking,centrifugation,and concentration of the supernatant,the residue was dissolved in 70%methanol,and interfering substances were removed through the Oasis®HLB cassette.Finally,it was rinsed with methanol,and detected with high performance liquid chromatography.Experimental results show that when 0.5 ppm,1 ppm,3 ppm,and 5 ppm are added to feed,the recovery rate is between 80.8%and 107.5%.When using only a photodiode detector,the minimum detection limit is 0.03 ppm;when using a mass spectrometer detector,the minimum detection limit can reach 0.005 ppm.The research results show that the developed high-performance liquid chromatography method for detecting diethylstilbestrol in feed is convenient,economical,and feasible,and can be used as an effective method for detecting diethylstilbestrol in feed.关键词
乙烯雌酚/高效能液相层析仪(HPLC)/光二极管侦测器(PDA)/饲料分析/液相层析质谱仪(LC/MS)Key words
diethylstilbestrol/high performance liquid chromatography(HPLC)/photodiode detector(PDA)/feed analysis/liquid chromatography mass spectrometer(LC/MS)
