中国美容医学2024，Vol.33Issue(5)：5-9,5.
非剥脱点阵激光联合侧柏叶酊对斑秃小鼠IL-7/IL-7Rα信号通路和Tregs细胞亚群的影响
Effects of Non-ablative Fractional Laser Combined with Platycladus Orientalis Tincture on IL-7/IL-7Rα Signaling Pathway and Tregs Cell Subsets in Alopecia Areata Mice
摘要
Abstract
Objective To investigate the therapeutic effect of 1 565 nm non-ablative fractional laser combined with Platycladus orientalis tincture(POT)on alopecia areata(AA)mice,and its impact on the IL-7/IL-7Rα signaling pathway and regulatory T cell(Treg)subsets.Methods Fifty adult male C3H/HeJ mice were randomly divided into control group(C group),model group(M group,induced with cyclophosphamide(CTX)to establish AA model),M+1565 nm group(treated with 1 565 nm non-ablative fractional laser for AA),M+POT group(treated with POT for AA),and M+1565 nm+POT group(treated with both 1 565 nm non-ablative fractional laser and POT for AA),10 mices in each group.Flow cytometry was used to detect the proportion of Treg subsets in the skin lesions of the C group and M group and in the peripheral blood mononuclear cells of all the groups.Western blot was used to detect the expression of IL-7 and IL-7Rα in the skin lesions of each group of mice.Results Compared with the C group,the expression of IL-7 and IL-7Rα in the skin lesions of the M group was significantly increased,while the proportion of Tregs cells was significantly decreased(P＜0.05).Compared with the M group,the expression of IL-7 in the M+1 565 nm group and M+POT group was decreased(P＜0.05).Compared with the M group,the expression of IL-7 and IL-7Rα in the M+1565 nm+POT group was decreased,and the proportion of Tregs cells was significantly increased(P＜0.05).Conclusion The combination of 1 565 nm non-ablative fractional laser combined with POT can inhibit the IL-7/IL-7Rα signaling pathway and reduce the proportion of Tregs cells in AA mice.关键词
1 565 nm非剥脱点阵激光/斑秃小鼠/侧柏叶酊/白细胞介素7/白细胞介素7受体α/调节性T细胞群Key words
1 565 nm non-ablative fractional laser/alopecia areata mice/Platycladus orientalis tincture/Interleukin 7/Interleukin-7 receptor α/regulatory T cell population分类
医药卫生引用本文复制引用
苏家光,黄家灿,罗世斌,陈信津,郑文军..非剥脱点阵激光联合侧柏叶酊对斑秃小鼠IL-7/IL-7Rα信号通路和Tregs细胞亚群的影响[J].中国美容医学,2024,33(5):5-9,5.基金项目
广西壮族自治区中医药管理局自筹经费科研课题(编号:Gzzc2020174) （编号:Gzzc2020174）
