脐带来源间充质干细胞抑制巨噬细胞M1极化OA北大核心CSTPCD
Umbilical cord-derived mesenchymal stem cells inhibit macrophage M1 polarization
目的:探究人脐带间充质干细胞(hUC-MSCs)对巨噬细胞M1/M2极化的作用.方法:将hUC-MSCs与佛波酯(PMA)诱导分化的巨噬细胞样细胞(pTHP-1巨噬细胞)共培养后进行转录组测序分析,筛选差异表达基因,进一步进行GO及KEGG富集分析.细胞增殖实验(CCK-8和EdU)分析hUC-MSCs对pTHP-1细胞增殖的影响.流式细胞术检测hUC-MSCs对LPS刺激的pTHP-1巨噬细胞炎症因子TNF-α表达及抑炎因子IL-10表达的影响…查看全部>>
Objective:To explore effect of human umbilical cord mesenchymal stem cells(hUC-MSCs)on macrophage M1/M2 polarization.Methods:hUC-MSCs were co-cultured with pTHP-1 cells which were macrophage-like cells induced by PMA and tran-scriptome sequencing data were analyzed.Differentially expressed genes were screened and analyzed by GO and KEGG enrichment analysis.Effect of hUC-MSCs on pTHP-1 cells proliferation was analyzed by cell proliferation assay(CCK-8 and EdU…查看全部>>
王晓旭;李超然;王惠;杨春娟;刘凤霞;徐栋花
潍坊医学院临床医学院,潍坊 261053潍坊医学院临床医学院,潍坊 261053潍坊医学院第一附属医院中心实验室,潍坊 261041潍坊医学院临床医学院,潍坊 261053潍坊医学院第一附属医院过敏反应科,潍坊 261041潍坊医学院第一附属医院中心实验室,潍坊 261041||潍坊医学院第一附属医院风湿免疫科,潍坊 261041
基础医学
脐带间充质干细胞pTHP-1转录组测序巨噬细胞极化
Umbilical cord mesenchymal stem cellspTHP-1Transcriptome sequencingMacrophagePolarization
《中国免疫学杂志》 2024 (4)
673-679,7
国家自然科学基金面上项目(82171790)山东省医药卫生科技发展计划(202103100273).
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