中国免疫学杂志2024，Vol.40Issue(4)：680-685,692,7.DOI:10.3969/j.issn.1000-484X.2024.04.002
miR-7抑制剂治疗MRL/lpr小鼠的实验性研究
Experimental study of miR-7 antagomir in treatment of MRL/lpr mice
摘要
Abstract
Objective:To investigate the efficacy of miR-7 antagomir in treatment of MRL/lpr mice.Methods:Thirty MRL/lpr mice were selected and randomly divided into miR-7 antagomir group,CTX group and Saline control group,with 10 mice in each group.MRL/lpr mice were treated at 13 weeks,and the above drugs were administered continuously for 5 weeks.General condition of the mice in each group was observed weekly and weighed,and serum and urine samples were collected.Serum ANA,anti-ds-DNA an-tibody and C3 levels of mice in the 13～17 weeks of three groups were detected by ELISA,and urinary protein level of mice in the above three groups were detected at every week.Changes in proportion of Tfh cells in the three groups of mice were detected by flow cytometry.RT-PCR was used to detect expression levels of miR-7 and PTEN mRNA in spleen and lymph nodes of three groups of mice.Pathological conditions of kidneys of three groups of mice were observed under light microscope by HE,PAS,PASM staining.Results:After 5 weeks of treatment,in terms of gross morphology:Compared with Saline group,mice in miR-7 antagomir group and CTX group were in good general condition,and their body weight were increased with the increased age.In terms of serology:Serum ANA and anti-ds-DNA antibody of miR-7 antagomir group and CTX group were significantly lower than those of Saline group,while level of complement C3 was significantly increased,there was no statistical difference in the above indicators between the two treatment groups.In terms of cytology:Proportion of Tfh cells in spleen of mice in miR-7 antagomir group was significantly lower than that in Saline group.Compared with Saline group,expression level of miR-7 in spleen B cells of mice in miR-7 antagomir group was decreased,while expression level of PTEN mRNA was increased.In addition,pathological changes of kidneys of mice in miR-7 antagomir group and CTX group were improved compared with Saline group,there was no significant difference in pathological changes of kidneys between the two groups.Conclusion:miR-7 can be used as a potential target for treatment of systemic lupus erythematosus,miR-7 antagomir can show similar therapeutic effects as the classical immunosuppressant CTX,effectively improve the lupus-like disease manifestations in MRL/lpr mice and delay the progression of the disease.关键词
系统性红斑狼疮/miR-7抑制剂/磷酸酶和张力蛋白同源物/环磷酰胺Key words
Systemic lupus erythematosus/miR-7 antagomir/PTEN/CTX分类
医药卫生引用本文复制引用
达古拉,王静,王勇,李鸿斌..miR-7抑制剂治疗MRL/lpr小鼠的实验性研究[J].中国免疫学杂志,2024,40(4):680-685,692,7.基金项目
国家自然科学基金项目(81660276) （81660276）
中央引导地方科技发展基金项目(2021ZY0013) （2021ZY0013）
内蒙古自然科学基金项目(2020MS08122) （2020MS08122）
内蒙古医科大学面上项目(YKD2021MS031,YKD2021MS028) （YKD2021MS031,YKD2021MS028）
内蒙古卫生健康委员会科技计划项目B类(202202187) （202202187）
内蒙古医科大学附属医院青年骨干项目(2023NYFYGG012) （2023NYFYGG012）
2022附院博士启动金项目(2022NYFYBS002). （2022NYFYBS002）
