摘要
Abstract
Objective:To investigate effect of long non-coding RNA(lncRNA)MAFG antisense RNA1(MAFG-AS1)target-ing miR-3180-3p on proliferation and apoptosis of oral squamous cell carcinoma.Methods:MAFG-AS1 and miR-3180-3p expressions in oral squamous cell carcinoma tissues and corresponding normal mucosa were detected by real-time quantitative PCR.Oral squamous cell carcinoma HSC4 was used as research object to construct oral squamous cell carcinoma lines that interfered with MAFG-AS1,overexpressed miR-3180-3p or miR-3180-3p inhibition.Cell viability,clonal formation numbers,apoptosis rate,cleaved cleaved-cas-pase 3 and cleaved-caspase 9 expressions were detected by CCK-8,plane cloning experiment,flow cytometry and Western blot,re-spectively.Targeting relationship between MAFG-AS1 and miR-3180-3p was assessed with luciferase reporter experiment.Results:Compared with normal mucosa,MAFG-AS1 expression was remarkably increased(P<0.05),while miR-3180-3p expression was re-markably decreased(P<0.05)in oral squamous cell carcinoma tissues.Interference with MAFG-AS1 or miR-3180-3p overexpression could reduce HSC4 cell viability and clonal formation numbers(P<0.05),promote cell apoptosis(P<0.05),and up-regulate expres-sions of cleaved-caspase 3 and cleaved-caspase 9 proteins(P<0.05).miR-3180-3p was direct target of MAFG-AS1,and MAFG-AS1 negatively regulates miR-3180-3p expression.miR-3180-3p inhibition could increase HSC4 cell viability and colony formation number(P<0.05),inhibit cell apoptosis(P<0.05),and down-regulate cleaved-caspase 3 and cleaved-caspase 9 protein expressions(P<0.05),thereby weakening effect of interference with MAFG-AS1 on proliferation inhibition and apoptosis promotion on HSC4 cells(P<0.05).Conclusion:Interfering with MAFG-AS1 can inhibit proliferation and promote apoptosis of oral squamous cell carcinoma cells HSC4 by up-regulating miR-3180-3p expression.关键词
MAFG-AS1/口腔鳞癌/miR-3180-3p/HSC4细胞/细胞增殖/凋亡Key words
MAFG-AS1/Oral squamous cell carcinoma/miR-3180-3p/HSC4 cells/Cell proliferation/Apoptosis分类
医药卫生
