毛华菊3种瓣型株系再生体系的建立OA北大核心CSTPCD

Chrysanthemum vestitum,a closely related hexaploid wild species of cultivated C.x morifolium has the same and typical flat,tubular and mixed floret forms in its natural population as cultivated chrysanthemum and is an ideal ma-terial for studying the petal variation in chrysanthemum.The development progress of this ray floret is affected by key differential genes such as auxin and floral organ development,but the lack of stable and efficient regeneration systems for different floret forms has restricted the study of petal type-related genes in C.vestitum.In this paper,the authors used three floret forms of C.vestitum collected from Funiu Mountain in Henan Province to establish regeneration systems using leaves and transverse thin cell layers(tTCLs)as explants.The results showed that the optimal media for callus induction and adventitious bud differentiation were MS+1 mg·L-1 NAA+2 mg·L-1 6-BA with flat floret leaves as explants,and the callus induction rate was 100％at 20 days after inoculation.The differentiation rate of adventitious buds was 100％.The optimal rooting medium was 1/2MS+0.2 mg·L-1 NAA,and the rooting rate of regenerated plants was 100％.The optimal leaf regeneration system for the flat floret strain was also suitable for determining both the tubular and mixed floret strains.The differentiation rates of adventitious buds were 83.46％and 91.67％,respectively,and the rooting rates were 100％.The flowering plants were observed after transplanting,and it was found that the flower types of the regenerated plants with three different floret forms obtained by the leaf regeneration system were stable,which provided a technical method for the subsequent analysis of the morphological variation mechanism of ray florets by using different floret forms of C.vestitum.