肾茶精水方对雄性SD大鼠生殖功能的保护机制OACSTPCD

Objective:To observe the protective mechanism of Shencha Jingshui formula(SJF)on reproductive function in rats.Methods:Seventy male SD rats were randomly divided into 7 groups:blank group,nephrotic syndrome(NS)group,NS + tripterygium wilfordii polyglycoside(TWP)group,NS + TWP + Wuzi Yanzong pill(WYP)group,and NS +TWP + low,medium and high dose SJF groups,with 10 rats in each group.Nephrotic syndrome model was established by injecting adriamycin into rat tail.Samples were collected after 30 days of gavage intervention.UTP,Alb,BUN,TC,SCr,TG were detected by automatic biochemical analyer.Serum levels of GnRH,E2,FSH,T and LH were detected by ELISA.The pathological changes of kidney and testis were observed by HE staining and Masson staining methods.Results:Compared with NS + TWP group,NS + TWP + WYP group,and NS + TWP + SJF of low,medium and high dose groups had significant improvements in reducing the atrophy of testicular spermatogenic tubules,reducing the vacuolation of lumen,increasing the number of spermatogenic cells in each layer.At the same time,the pathological changes such as glomerular sclerosis,renal tubule injury,renal interstitial fibrosis and inflammatory cell infiltration were also significantly reduced.Compared with NS group,GnRH,E2,T and Alb in NS + TWP + WYP group increased,while FSH,LH,BUN,SCr,TC,UTP and TG decreased(P<0.05).The levels of FSH,BUN,SCr and TG decreased and the content of Alb increased in NS +TWP +medium dose SJF group(P<0.05);the contents of BUN,SCr,TC,UTP and TG decreased and the content of Alb increased in NS + TWP + high dose SJF group(P<0.05);Compared with NS +TWP group,the levels of GnRH,E2,T and Alb in NS +TWP +WYP group increased,the levels of FSH and LH decreased(P<0.05),and the levels of Alb in NS + TWP + high-dose SJF group increased(P<0.05).Conclusion:The effect of high dose SJF is similar to that of WYP,which can effectively treat nephrotic syndrome and have a positive effect on male infertility.This study provides a new therapeutic idea for the simultaneous treatment of nephrotic syndrome and male infertility.