不同提取方法对路路通活性成分及抗氧化性的比较研究OACSTPCD

Objective:To study the effects of reflux extraction,enzymolysis extraction and ultrasonic extraction on gallic acid content of sweetgum fruit,and compare its antioxidant activities.Methods:Orthogonal method was used to optimize the extraction conditions of the reflux extraction method and enzymolysis extraction method of sweetgum fruit;the ultrasonic extraction method was used according to the reference,and the effects of the three methods on the extraction rate of gallic acid in sweetgum fruit under the best extraction conditions were compared.The extraction solution's ability to scavenge ABTS,DPPH,hydroxyl radicals,and reduce Fe3+ was investigated,and the effects of different extraction methods on the antioxidant activity of sweetgum fruit were compared.Results:The optimal conditions for the reflux extraction method were 50%ethanol by volume,1h reflux time,and 1∶40(g/mL)liquid-to-material ratio.The optimal extraction process conditions for the enzymolysis extraction method were 0.6 g of enzyme,50 min of enzymolysis time,and 1∶30(g/mL)of liquid to material ratio.The extraction rates of gallic acid in sweetgum fruit were 24.63%,20.08%,and 13.58%,respectively,using reflux extraction,enzymatic extraction,and ultrasonic extraction methods.Sweetgum fruit extracts by all three extraction methods had good antioxidant capacity.The scavenging ability of ABTS,hydroxyl radicals and Fe3+ reduction ability were positively correlated with the concentration.The DPPH method is not related to concentration.However,there is a clear DPPH eliminating ability of extracts from different extraction methods.When the concentration of gallic acid was 5.4 mg/mL,the scavenging rates of ABTS capacity of the extracts by reflux extraction,enzymolysis extraction and ultrasonic extraction reached 97.01%,81.00%and 95.60%,respectively;when the concentration of gallic acid was 9.0 mg/mL,the scavenging rates of hydroxyl radicals reached 88.11%,51.00%and 78.73%,respectively,and the scavenging rate of Fe3+ reduction capacity reached 3.770,3.021,3.637,respectively.When the gallic acid concentration in sweetgum fruit was 3.6 mg/mL,the extracts by reflux extraction,enzymolysis extraction and ultrasonic extraction achieved 90.15%,89.36%and 90.06%scavenging of DPPH free radicals,respectively.Conclusion:The reflux extraction method can effectively extract the active ingredient gallic acid from sweetgum fruit.The higher the content of gallic acid,the stronger the antioxidant capacity of Sweetgum Fruit extract.