大连工业大学学报2024，Vol.43Issue(2)：84-89,6.DOI:10.19670/j.cnki.dlgydxxb.2024.0202
葛根素微球的制备及其对2型糖尿病肝损伤的保护作用
Preparation of puerarin microspheres and their protective effect on liver injury in type 2 diabetes mellitus
摘要
Abstract
An oral puerarin microsphere(alginate supported puerarin drug-carrying gel microsphere)was developed by electrostatic spray technology.Optical microscopy and scanning electron microscopy results showed that the sphericity and dispersibility of puerarin microspheres were high,with an average particle size of(391.62±22.06)μm.The release curve in vitro showed that puerarin microspheres could avoid gastric acid decomposition and release to the intestine slowly,thereby increasing the bioavailability of puerarin.After 10 weeks of administration of puerarin microspheres to mice,the blood glucose levels in the puerarin microspheres group was decreased by approximately 31.57％and the liver index was decreased by 18.67％compared with the model group.Masson staining result showed that the degree of liver fibrosis could be effectively reduced by puerarin microspheres and liver function are improved.The expression of glycogen synthesis related proteins GSK-3β and GYS-2 in mice liver in each group were detected by Western Blot.Compared with the model group,the expression of GSK-3β in the puerarin microsphere group was decreased by 69.33％,while the expression of GYS was increased by 126.19％.The results showed that oral administration of puerarin microspheres could effectively promote the synthesis of liver glycogen,reduce blood sugar,and thus alleviate the symptoms of type 2 diabetes and the liver injury caused by type 2 diabetes.关键词
葛根素/凝胶微球/2型糖尿病/糖尿病肝损伤Key words
puerarin/gel microspheres/type 2 diabetes/diabetic liver injury分类
轻工纺织引用本文复制引用
李思慧,李燕飞,闫慧佳,胡蒋宁..葛根素微球的制备及其对2型糖尿病肝损伤的保护作用[J].大连工业大学学报,2024,43(2):84-89,6.基金项目
国家自然科学基金项目(32172208). （32172208）
