大连工业大学学报2024，Vol.43Issue(2)：96-101,6.DOI:10.19670/j.cnki.dlgydxxb.2024.0204
大麦中抑制镰刀霉菌的乳酸菌筛选及其在制麦中的应用
Screening of Lactiplantibacillus plantarum inhibiting Fusarium graminearum in barley and its application in malting
摘要
Abstract
There are various microorganisms on the surface of barley,among which Fusarium graminearum and its secreted mycotoxins can affect the malting process and malt quality.To eliminate the adverse effects of F.graminearum,a strain with significant inhibitory effect was isolated and screened from the surface of barley using MRS selective culture medium.Based on morphological observation,physiological and biochemical experiments,and 16S rDNA gene sequence analysis,the strain was identified as Lactiplantibacillus plantarum named BY66.During the wheat making process,the biomass and deoxynivalenol content of F.graminearum could be reduced by 56.2％and 79.1％,respectively,through adding 0.5 mL/kg of BY66 fermentation broth.The negative effects of F.graminearum on barley germination and malt quality also could be eliminated by adding BY66 fermentation broth.Compared with the infected barley,the germination rate of barley could be increased by 31％with the addition of 0.5 mL/kg BY66 fermentation broth.关键词
制麦/植物乳杆菌/禾谷镰刀霉菌/真菌毒素Key words
malting/Lactiplantibacillus plantarum/Fusarium graminearum/mycotoxins分类
生物科学引用本文复制引用
王羽,孙笑天,富洋,俞志敏,杨帆,佟晔..大麦中抑制镰刀霉菌的乳酸菌筛选及其在制麦中的应用[J].大连工业大学学报,2024,43(2):96-101,6.基金项目
国家自然科学基金项目(31671796) （31671796）
辽宁省教育厅高等学校基本科研项目(LJKQZ2021114) （LJKQZ2021114）
大连市重点研发计划(2022YF16SN064). （2022YF16SN064）
