大连工业大学学报2024，Vol.43Issue(2)：108-113,6.DOI:10.19670/j.cnki.dlgydxxb.2024.0206
仿刺参过氧化氢酶的理化性质
Physical and chemical properties of Apostichopus japonicus catalase
摘要
Abstract
A recombinant catalase(rAj-CAT)with antioxidant activity was expressed by the recombinant engineering bacterium BL21(DE3)/pET-28c-Aj-CAT.The recombinant protein rAj-CAT exhibited a characteristic peak spectrum at 405 nm,which was not affected by Na2S2O4.Typical catalase inhibitors such as 3-amino-1,2,4-triazole,hydroxylamine hydrochloride and sodium azide have significant inhibitory effects on rAj-CAT.The results indicated that the catalase was a typical single functional catalase.The optimal enzyme activity conditions for rAj-CAT were tested.The result showed that rAj-CAT had the ability to degrade H2O2 in a lower temperature range(4-30 ℃),with an optimal enzyme activity temperature of 10 ℃.The rAj-CAT has a wide pH tolerance range and exhibits catalytic activity from pH 4.0 to 11.0.The rAj-CAT can also tolerate various metal ions and chemicals,including Triton X-100,Tween 20 and EDTA.But DTT,β-ME,SDS,chymotrypsin and trypsin had strong destructive effects on the activity of rAj-CAT to hydrolyze H2O2.The results indicated that Aj-CAT of Apostichopus japonicus is a catalase with high activity at lower temperatures and stable enzymatic properties.关键词
仿刺参/过氧化氢酶/酶类型Key words
Apostichopus japonicas/catalase/enzyme type分类
生物科学引用本文复制引用
简凡杰,刘影,齐洪庆,邢萱,李成..仿刺参过氧化氢酶的理化性质[J].大连工业大学学报,2024,43(2):108-113,6.基金项目
辽宁省自然科学基金项目(2021-MS-302). （2021-MS-302）
