儿科药学杂志2024，Vol.30Issue(5)：1-5,5.DOI:10.13407/j.cnki.jpp.1672-108X.2024.05.001
五味子苷B调控miR-370-3p/CCL3轴对幼年肺炎小鼠免疫炎症因子水平的影响
Impacts of Schisandrin B on Immune Inflammatory Factors in Juvenile Mice with Pneumonia by Regulating miR-370-3p/CCL3 Axis
摘要
Abstract
Objective:To investigate the mechanism of Schisandrin B(Sch B)in juvenile mice with pneumonia.Methods:Ninety juvenile male mice were randomly divided into the control group,model group,Sch B low dose group(Sch BL group),Sch B high dose group(Sch BH group),Sch BH+antagomir-NC group and Sch BH+miR-370-3p antagomir group,with 15 mice in each group.Sch BL group and Sch BH group were given 20 and 60 mg/kg Sch B,respectively.For the Sch BH+antagomir-NC group and Sch BH+miR-370-3p antagomir group,1 nmol of miR-370-3p antagomir and antagomir-NC were first dissolved in 20 μL of phosphate buffer solution(PBS),and Sch B was gavaged,miR-370-3p antagomir,antagomir-NC plasmid were injected into mice by tail vein,respectively.The control group and model group were given the same amount of normal saline.The drug was administered once a day for 7 d.The wet to dry weight ratio of pulmonary tissue of mice in each group was measured.Hematoxylin Eosin(HE)staining was applied to observe the pathological morphology of pulmonary tissue in each group of mice.Levels of inflammatory factors in pulmonary tissues of mice were detected.Flow cytometry was applied to detect T lymphocyte subsets in peripheral blood.Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the levels of miR-370-3p and CCL3 mRNA in the pulmonary tissues.Double Luciferase experiment was applied to verify the targeting relationship between miR-370-3p and CCL3.Results:Compared with the control group,the pulmonary tissue structure of juvenile mice in the model group was disordered,with thickened alveolar walls,a large number of inflammatory cell infiltration,and severe tissue damage,the value of wet to dry weight,percentage of CD8+,levels of tumour necrosises factor(TNF)-α,interleukin(IL)-6 and CCL3 mRNA increased,CD4+,CD4+/CD8+,and miR-370-3p decreased(P＜0.05).Compared with the model group,the alveolar wall in pulmonary tissue of juvenile mice in Sch BL group and Sch BH group became thinner,inflammatory cell infiltration reduced significantly,and damage was alleviated,the value of wet to dry weight,CD8+,levels of TNF-α,IL-6 and CCL3 mRNA decreased,CD4+,CD4+/CD8+,and miR-370-3p increased(P＜0.05).The addition of miR-370-3p antagomir for compensatory experiments showed that the protective effects of Sch B on immune function and inflammation in juvenile mice with pneumonia were reversed,and the level of CCL3 mRNA increased(P＜0.05).Double Luciferase reporter gene experiment verified that there was a targeting relationship between miR-370-3p and CCL3.Conclusion:Sch B can enhance the immune function of juvenilea mice with pneumonia and inhibit the inflammatory response by targeting the miR-370-3p/CCL3 axis.关键词
五味子苷B/miR-370-3p/CCL3轴/肺炎/免疫功能/炎症Key words
Schisandrin B/miR-370-3p/CCL3 axis/pneumonia/immune function/inflammation分类
医药卫生引用本文复制引用
杨翠玲,高军铭,董丽君,王丽敏,张永峰..五味子苷B调控miR-370-3p/CCL3轴对幼年肺炎小鼠免疫炎症因子水平的影响[J].儿科药学杂志,2024,30(5):1-5,5.基金项目
山东省中医药科技发展计划项目,编号2019-0031. （）
