福建农业学报2024，Vol.39Issue(2)：137-146,10.DOI:10.19303/j.issn.1008-0384.2024.02.003
木薯MebZIP2基因克隆及其功能分析
Gene Cloning and Functional Analysis of MebZIP2 in Cassava
摘要
Abstract
[Objective]A functional analysis was conducted on the cloned bZIP transcription factor in cassava.[Method]The coding sequence(CDS)of ZIP2 in Cassava SC205 was amplified to perform a bioinformatic analysis in determining the subcellular localization and expression of MebZIP2.A pNC-green-subN fusion expression vector was constructed and transferred into tobacco epidermal cells using the Agrobacterium-mediated method.Fluorescence signals were observed for subcellular localization of the protein.Expressions of MebZIP2 in tissues at different stages of storage root development of the plant were determined.Interactions between MebZIP2 and the promoters of starch synthesis genes were analyzed by the yeast one-hybrid assay.[Result]The CDS of MebZIP2 was 465 bp long encoded 154 amino acids with a molecular weight of 17 891.35 Da and an isoelectric point of 5.23.The unstable hydrophilic protein contained a bZIP-conserved domain with the highest sequence similarity to that of one of Hevea brasiliensis at 74.22%.The promoter region of MebZIP2 included responsive elements to light,hormones(such as gibberellin,salicylic acid,and jasmonic acid),endosperm expression,and stresses.The protein localized in the cell membrane as well as the nucleus.The gene expressed highly in the root apical meristems,fibrous roots,stems,and at early development stages of storage roots and co-expressed with those involved starch synthesis,such as MeAPL5a,MeGBSS1,and MeISA1,whose promoters could interact with the protein.[Conclusion]Belonging to the bZIP family associated with plant growth,development,starch synthesis,and resistance to abiotic stress,MebZIP2 exhibited a tissue-and root development stage-specific expression.The protein interacted with the promoters of MeAPL5a,MeGBSS1,and MeISA1 related to starch synthesis in the plant.It might also be involved in cassava storage root development.关键词
木薯/bZIP转录因子/MebZIP2/基因克隆/功能分析Key words
Cassava/bZIP transcription factor/MebZIP2/cloning/functional analysis分类
农业科技引用本文复制引用
陈甘露,颜彦,孟宪伟,付莉莉,邱先进,丁泽红,胡伟..木薯MebZIP2基因克隆及其功能分析[J].福建农业学报,2024,39(2):137-146,10.基金项目
国家自然科学基金项目(32260486) （32260486）
三亚崖州湾科技城科技专项(SCKJ-JYRC-2023-68) （SCKJ-JYRC-2023-68）
中国热带农业科学院基本科研业务费专项(1630052023004) （1630052023004）
海南省重点研发计划项目(ZDYF2022XDNY259) （ZDYF2022XDNY259）
