分析化学2024，Vol.52Issue(4)：469-480,12.DOI:10.19756/j.issn.0253-3820.231359
基于CRISPR/Cas的生物传感器在食源性病原菌检测中的应用进展
Advances of CRISPR/Cas-based Biosensor in Detection of Food-Borne Pathogens
摘要
Abstract
Rapid and accurate detection methods for food-borne pathogens are essential to ensure food safety and human health.One promising innovation in this area is the clustered regularly interspaced short palindromic repeats/CRISPR-associated systems(CRISPR/Cas)biosensor,which utilizes Cas protein and CRISPR RNA(crRNA)ribonucleo protein to specifically recognize target genes,and converts target signals into detectable physical and chemical signals.The CRISPR/Cas biosensor shows many advantages,such as high specificity,programmability,and ease of use,making it promising to pathogen detection.This paper introduced the principles and characteristics of CRISPR/Cas systems,along with the strategies for signal recognition,amplification,and output based on different CRISPR/Cas biosensors,and their respective applications in food-borne pathogen detection.Furthermore,the construction principles and challenges of multiple biosensors based on CRISPR/Cas were explored,as well as their potential for simultaneous detection of multiple pathogens.Finally,the challenges and future development trends of CRISPR/Cas-based biosensors in rapid pathogen detection were discussed,aiming to provide valuable reference and inspiration for biosensor designers and food safety practitioners.关键词
食源性病原菌/CRISPR/Cas/生物传感器/评述Key words
Food-borne pathogens/CRISPR/Cas/Biosensor/Review引用本文复制引用
张孝渊,姚志豪,何开雨,王洪梅,徐霞红,吴祖芳,王柳..基于CRISPR/Cas的生物传感器在食源性病原菌检测中的应用进展[J].分析化学,2024,52(4):469-480,12.基金项目
国家自然科学基金项目(No.32172307)和浙江省基础公益研究计划项目(No.LGN21C200007)资助. Supported by the National Natural Science Foundation of China(No.32172307)and the Basic Public Welfare Research Program of Zhejiang Province,China(No.LGN21C200007). （No.32172307）
