福建农业学报2024，Vol.39Issue(2)：165-174,10.DOI:10.19303/j.issn.1008-0384.2024.02.006
百香果YUCCA基因家族鉴定与表达分析
Identification and Expressions of YUCCA Family in Passiflora edulis
摘要
Abstract
[Objective]Bioinformatics of YUCCA family encoding the flavin-containing monooxygenase associated with biosynthesis of indole-3-acetic acid(IAA)in passion fruit was studied.[Methods]Bioinformatic methods were applied to analyze the physicochemical properties,conserved domains,chromosome location,structure,phylogenetic tree,and cis-acting elements of the genes in Passiflora edulis Sims.qRT-PCR was used to determine the expressions of some members under IAA treatment.[Results]There were 29 YUCCA members unevenly distributed in 8 chromosomes of P.edulis.They significantly differed in length that ranged from 552 bp to 9210 bp and contained 1-8 introns and 8 conserved motifs.A phylogenetic tree analysis divided the family into three distinct categories,and within a same class the members were highly conservative.Genetically,the genes were more closely related to Medicago sativa L.and Arabidopsis thaliana than Oryza sativa L.The cis-acting element analysis indicated that the promoter of the family genes could be induced by various hormones and respond to various stresses.PeYUCCA6,PeYUCCA11,and PeYUCCA16 showed low or no expression in the leaves of Tainong and Golden Passion Fruit,but PeYUCCA23 had a high expression suggesting its predominant role in the plant development.The treatment of 100 μmol·L-1 IAA significantly elevated the expressions of PeYUCCA7,PeYUCCA13,PeYUCCA17,PeYUCCA24,and PeYUCCA26.[Conclusion]The expressions of YUCCAsin P.edulis varied greatly under IAA treatment.But as a family,the genes likely played an important role in the growth,development,and resistance to adverse environment of passion fruits.关键词
百香果/吲哚-3-乙酸/YUCCA基因家族/生物信息学/定量分析Key words
Passiflora edulis Sims/IAA/YUCCA family/bioinformatics/quantitative analysis分类
农业科技引用本文复制引用
潘佳怡,潘若云,江文洁,任锐,方庭..百香果YUCCA基因家族鉴定与表达分析[J].福建农业学报,2024,39(2):165-174,10.基金项目
福建省自然科学基金项目(2021J05022) （2021J05022）
福建省种业创新与产业化工程项目(zycxny2021010) （zycxny2021010）
福建省高原学科建设项目(102/71201801101) （102/71201801101）
