分析化学2024，Vol.52Issue(4)：481-491,11.DOI:10.19756/j.issn.0253-3820.221448
RNA荧光适配体在生物传感与成像中的应用研究进展
Application Progress of RNA Fluorescence Aptamers in Biosensing and Imaging
摘要
Abstract
RNA fluorescence aptamers are RNA sequences that can specifically bind to non-toxic,cell permeable,and self-fluorescent target molecules and activate their luminescent properties.These aptamers provide powerful tools for biosensing and imaging researches due to their simple structure,easy synthesis,and easy transfection.This article summarized the characteristics and development history of various RNA fluorescent aptamers,including Malachite Green,Spinach,Broccoli,Mango,Corn,and Pepper family,as well as their corresponding fluorescent groups.The applications of RNA fluorescent aptamers were also reviewed from two aspects:extracellular detection and cell imaging.This review might provide guidance for labeling,detection and interactions of molecules from proof of concept and clinical assessment to practical clinical and biomedical applications.关键词
荧光适配体/RNA/生物传感/生物成像/评述Key words
Fluorescent aptamers/RNA/Biosensing/Bioimaging/Review引用本文复制引用
邱星晨,范存霞,白瑞,谷雨,李长明,郭春显..RNA荧光适配体在生物传感与成像中的应用研究进展[J].分析化学,2024,52(4):481-491,11.基金项目
国家自然科学基金项目(Nos.21972102,21904092)和国家重点研发计划项目(No.2021YFA0910400)资助. Supported by the National Natural Science Foundation of China(Nos.21972102,21904092)and the National Key Research and Development Program of China(No.2021YFA0910400). （Nos.21972102,21904092）
