南方农业学报2024，Vol.55Issue(2)：378-387,10.DOI:10.3969/j.issn.2095-1191.2024.02.008
黄连素与聚β-羟基丁酸酯对大口黑鲈生长、免疫和肠道菌群的影响
Effects of berberine and poly-β-hydroxybutyrate on growth,immunity and intestinal flora of largemouth bass(Micropterus salmoides)
摘要
Abstract
[Objective]To investigate the effects of adding berberine(BBR)and poly-β-hydroxybutyrate(PHB)to feed on the growth,immunity and intestinal flora of largemouth bass(Micropterus salmoides)in the inverse-season fry.So as to provide theoretical basis for the application of BBR and PHB in the feed for largemouth bass and the inverse-season fry breeding of largemouth bass.[Method]Largemouth bass inverse-season fry(body mass 130.34±0.26 mg)were used as test materials,and fed basic feed as control(CK),feed B(1‰ BBR),feed P(1‰ PHB),feed BP(1‰ BBR+ 1‰ PHB)in B,P and MIX groups respectively,and BP5,BP7 and BP14 groups were fed with feed B and feed P alter-nately at the frequency of every 5,7 and 14 d.The largemouth bass was cultured for 30 d.The growth performance,liver and intestinal biochemical indexes were measured,and the intestinal flora structure and function were analyzed.[Result]In growth performance,compared with CK,the final total mass was significantly higher(P<0.05,the same below),and the feed coefficient was significantly decreased in all treatment groups except P and MIX groups;the weight gain rate and survival rate in the BP5,BP7 and BP14 groups were significantly higher than that in CK;the final total mass,survival rate and weight gain rate were the highest in BP7 group while the feed coefficient was the lowest.In the liver,ACP and AKP activities were significantly higher in the B,P,BP5,BP7,BP14 and MIX groups than in CK,while GOT and GPT activities were significantly lower than CK.In the intestine,compared with CK,the contents of MDA and IL-6 were sig-nificantly lower in other treatment groups,while the contents of CAT and LZM were significantly increased;the content of TNF in BP5 and BP7 groups was significantly lower than that in CK.At the phylum level,the relative abundance of Proteobacteria was decreased and that of Firmicutes was increased in the BP5,BP7 and MIX groups,relative abundance of Bacteroidota in the BP7 group increased obviously compared with CK.At the genus level,compared with CK,the rela-tive abundance of Achromobacter decreased greatly and that of Lactobacillus increased greatly in the BP5 and BP7 groups.Microbial functional genes were significantly enriched in metabolic pathway and environmental information pro-cessing pathway in BP7 group.[Conclusion]Adding BBR to the feed can improve the growth performance of largemouth bass in autumn,and the addition or mixing of BBR and PHB can effectively improve the immunity and antioxidant ability of the fish,improve the liver damage,and reduce the intestinal inflammatory response,and the best effect is achieved by alternating the feeding of BBR-containing feeds with PHB-containing feeds at a frequency of eve-ry 7 d.It is assumed that BBR and PHB change the structure and function of largemouth bass intestinal flora and improve liver and intestinal health关键词
黄连素(BBR)/聚β-羟基丁酸酯(PHB)/大口黑鲈/生长/免疫/肠道菌群Key words
berberine(BBR)/poly-β-hydroxybutyrate(PHB)/largemouth bass(Micropterus salmoides)/growth/immunity/intestinal flora分类
农业科技引用本文复制引用
刘雅荣,夏耘,魏东,张凯,谢骏,王广军,龚望宝,郁二蒙,孙金辉..黄连素与聚β-羟基丁酸酯对大口黑鲈生长、免疫和肠道菌群的影响[J].南方农业学报,2024,55(2):378-387,10.基金项目
国家自然科学基金青年科学基金项目(32002401) （32002401）
广东省基础与应用基础研究基金项目(2020A1515110249) Youth Project of National Natural Science Foundation of China(32002401) （2020A1515110249）
Guangdong Basic and Applied Basic Research Foundation(2020A1515110249) （2020A1515110249）
