分析化学2024，Vol.52Issue(4)：550-558,9.DOI:10.19756/j.issn.0253-3820.241038
基于磁性多壁碳纳米管的磁性固相萃取-超高效液相色谱-串联质谱法测定农产品中22种真菌毒素
Magnetic Multi-Walled Carbon Nanotubes Nanomaterials as Magnetic Solid Phase Extraction Adsorbents for Simultaneous Determination of Multiple Mycotoxins in Agricultural Products by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A simple,rapid and efficient magnetic solid-phase extraction(MSPE)method was established by employing magnetic multi-walled carbon nanotubes(Fe3O4-MWCNTs)as adsorbents.And further,by coupling with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),an accurate quantitative approach for analysis of 22 kinds of mycotoxins in agricultural products was developed.With Fe3O4-MWCNTs as MSPE adsorbents,the enrichment efficiency of various target fungal toxins was improved and the sample pretreatment time was reduced effectively.In the pre-treatment stage,the extraction solution,adsorbent type,adsorbent dosage and elution solution type were systematically optimized.Under the optimal pre-treatment conditions of MSPE,the methodological investigation of the method was carried out.Under the optimal conditions,the proposed method showed a good linearity(R2≥0.9966),high sensitivity(The limits of detection(S/N=3)were 0.0008-1.6337 ng/g and the limits of quantification(S/N=10)were 0.0025-5.4457 ng/g),adequate recoveries(71.5%-118.4%),and good repeatability(Intra-day precision in the range of 1.3%-10.9%and inter-day precision in the range of 2.3%-11.6%).The improved MSPE-UPLC-MS/MS analytical method had advantages inculding simplicity,rapidity and high efficiency,and could realize simultaneous detection of multiple mycotoxins in large quantities of agricultural products.关键词
真菌毒素/磁性多壁碳纳米管/磁性固相萃取/超高效液相色谱-串联质谱Key words
Mycotoxins/Magnetic multi-walled carbon nanotubes/Magnetic solid-phase extraction/Ultra-high performance liquid chromatography-tandem mass spectrometry引用本文复制引用
马帅,梁刚,满燕,靳欣欣,李安..基于磁性多壁碳纳米管的磁性固相萃取-超高效液相色谱-串联质谱法测定农产品中22种真菌毒素[J].分析化学,2024,52(4):550-558,9.基金项目
国家自然科学基金项目(No.32202203)和北京市农林科学院青年科研基金项目(No.QNJJ202245)资助. Supported by the National Natural Science Foundation of China(No.32202203)and the Youth Research Fund of Beijing Academy of Agriculture and Forestry(No.QNJJ202245). （No.32202203）
