miR-454-3p通过靶向STAT3调控CNE-2人鼻咽癌细胞上皮-间质转化OA北大核心CSTPCD

Objective:To explore the levels of miR-454-3p and STAT3 in nasopharyngeal carcinoma(NPC)tissues,as well as the effect of miR-454-3p targeting STAT3 on the process of epithelial-mesenchymal transition(EMT)in NPC cells.Methods:NPC tissue and healthy tissue were collected in five cases from the Affiliated Hospital of Youjiang Medical University for National-ities and Baise People's Hospital from June to August 2023.qRT-PCR detected the expression of miR-454-3p and STAT3 in each tissue sample.Untreated NPC cells CNE-2 was used as a blank control group(Blank group).miR-454-3p mimics,mimics NC(negative control),miR-454-3p inhibitor and inhibitor NC(negative control)were transfected into CNE-2 cells by Lipofectami-neTM 3000 as miR-454-3p mimics group,mimics NC group,miR-454-3p inhibitor group and inhibitor NC group.The targeting re-lationship of miR-454-3p and STAT3 was predicted by Targetscan and confirmed by the dual-luciferase assay.The transfection ef-ficiency and STAT3 mRNA levels were assessed using qRT-PCR following transfection.Wound healing and transwell experi-ments were executed to assess cell migratory and invasive abilities.The expression levels of STAT3 and proteins associated with EMT were assessed using Western blot analysis.Results:The expression of miR-454-3p was reduced in NPC tissues relative to healthy tissues,while the expression of STAT3 was elevated(all P<0.001).The dual-luciferase assay findings indicated that miR-454-3p targeting regulates expression of STAT3(P<0.001).Compared to both the blank group and mimics NC group,STAT3,N-cadherin,and Vimentin expressions of the miR-454-3p mimics group were reduced.Conversely,it exhibited an in-crease in E-cadherin expression.The migratory and invasive capabilities of the cells were weakened(all P<0.05).Compared to the blank group and inhibitor NC group,the expressions of STAT3,N-cadherin,and Vimentin in the miR-454-3p inhibitor group were upregulated.In contrast,the expression of E-cadherin was downregulated.Cells showed both enhanced migratory and inva-sive capacity(all P<0.05).Conclusion:In NPC tissues,miR-454-3p demonstrated downregulation,whereas STAT3 exhibited upregulation.Notably,within CNE-2 human NPC cells,miR-454-3p significantly reduced STAT3 activity and effectively sup-pressed cell invasion and migration.