海洋科学2024，Vol.48Issue(2)：69-78,10.DOI:10.11759/hykx20220726002
一株海洋红法夫酵母Phaffia rhodozyma RP-306产虾青素的发酵条件优化
Optimization of fermentation conditions for astaxanthin production by marine Phaffia rhodozyma RP-306
摘要
Abstract
To improve the yield of astaxanthin produced by Phaffia rhodozyma RP-306,fermentation conditions were optimized through single-factor and response surface methodology.Moreover,a single-factor experiment was designed for each factor during fermentation.First,initial values of six factors,including sucrose,yeast powder,ammonium sulfate,pH,temperature,and liquid volume,were determined by a single-factor experiment.Second,the Plackett-Burman design was employed to screen out influential factors,such as sucrose,pH,and temperature.The steepest climbing experiment was utilized to determine the reasonable range of the three factors.Finally,optimal fermentation conditions were obtained by central composite design in response surface methodology.The final re-sults were as follows:sucrose 29.93 g/L,yeast powder 2.0 g/L,ammonium sulfate 5.0 g/L,pH 5.27,and tempera-ture 19.9℃.Under these conditions,astaxanthin yield increased from 13.46 to 17.04 mg/L,which was 26.61%higher than that before optimization.Moreover,astaxanthin yield reached 28.86 mg/L via batch fermentation in a 5.0-L fermenter,which encouraged further fermentation scale-up research.关键词
红法夫酵母/虾青素/发酵条件/响应面优化Key words
Phaffia rhodozyma RP-306/astaxanthin/fermentation conditions/response surface optimization分类
生物科学引用本文复制引用
林嘉祺,庄岩,刘骁,曾翰庭,张玉苍,产竹华,曾润颖..一株海洋红法夫酵母Phaffia rhodozyma RP-306产虾青素的发酵条件优化[J].海洋科学,2024,48(2):69-78,10.基金项目
自然资源部资助项目(HX05-210101-21) （HX05-210101-21）
自然资源部新专项资助项目(HR02-200301-22)[Ministry of Natural Resources Funded Project,No.HX05-210101-21 （HR02-200301-22）
Ministry of Natural Resources New Special Funded Project,No.HR02-200301-22] （）
