精准医学杂志2024，Vol.39Issue(2)：108-113,119,7.DOI:10.13362/j.jpmed.202402003
低聚木糖对高脂饮食致脂肪肝小鼠的保护作用及其机制
Protective effect of xylo-oligosaccharides on mice with fatty liver induced by high-fat diet and related mechanisms
摘要
Abstract
Objective To investigate the protective effect of xylo-oligosaccharides on mice with fatty liver induced by high-fat diet and related mechanisms.Methods A total of 40 male C57/BL6J mice,aged 5 weeks,were randomly divided into low-fat diet control group(LFD group),high-fat diet model group(HFD group),high-fat diet+low-dose xylo-oligosaccharide in-tervention group(XOS.L group),and high-fat diet+high-dose xylo-oligosaccharide intervention group(XOS.H group),with 10 mice in each group.The mice in the LFD group were fed a low-fat diet containing 10％fat,and those in the HFD group were fed a high-fat diet containing 60％fat;in addition to the treatment in the HFD group,the mice in the XOS.L group were given xylo-oligosaccharides at a dose of 0.35 g/kg by gavage once a day,and those in the XOS.H group were given xylo-oligosaccharides at a dose of 1.0 g/kg by gavage once a day;the mice in the LFD group and the HFD group were given an equal volume of normal saline by gavage.At the end of week 16,blood,liver tissue,and fecal samples were collected from each group of mice;the serum levels of alanine ami-notransferase(ALT),aspartate aminotransferase and triglyceride(TG)were measured;HE staining and oil red O staining were used to perform a histological evaluation of the liver;16S rRNA microbial sequencing technology and metabolomics were used to measure the changes in fecal gut microbiota and metabolites.Results compared with LFD group,the HFD group showed significant steatosis of the liver,disturbances in various metabolites including arachidonic acid and linoleic acid(LA),sig-nificant increases in the abundances of the pathogenic bacteria such as Faecalibaculum,Bacteroidaceae,and significant reductions in the abundances of the probiotic bacteria such as Rurinococcaceae,Bacteroides.Compared with the HFD group,the XOS.H groups showed a significant improvement in liver steatosis and the abundances of related microbiota such as Ruminococcaceae and Actinobacteria,significant reductions in liver SAF scores and the enrichment intensities of the proinflammatory metabolites such as Prostaglandin H2,and significant increases in the enrichment intensities of the metabolites such as Prostaglandin D2,LA,and do-cosahexaenoic acid.Conclusion Xylo-oligosaccharides can significantly improve liver steatosis,gut microbiota dysbiosis,and metabolite disturbances induced by high-fat diet in mice,possibly by regulating the gut microbiota to interfere with the metabolic pathways such as arachidonic acid and LA.关键词
寡糖类/益生元/非酒精性脂肪性肝病/胃肠道微生物组/脂类代谢/代谢网络和途径Key words
Oligosaccharides/Prebiotics/Non-alcoholic fatty liver disease/Gastrointestinal microbiome/Lipid metabolism/Metabolic networks and pathways分类
医药卫生引用本文复制引用
李翰卿,李宇鹍,张震,田字彬,齐兴四..低聚木糖对高脂饮食致脂肪肝小鼠的保护作用及其机制[J].精准医学杂志,2024,39(2):108-113,119,7.基金项目
国家自然科学基金面上项目(81970461) （81970461）
