激光生物学报2024，Vol.33Issue(2)：160-166,7.DOI:10.3969/j.issn.1007-7146.2024.02.008
柯萨奇病毒A组10型VP1蛋白线性中和表位的筛选和鉴定
Screening and Identification of Linear Epitopes on the VP1 Protein of Coxsackievirus A10
摘要
Abstract
Coxsackievirus A10(CV-A10)is one of the most common pathogens causing hand,foot and mouth disease(HFMD).The 39 candidate epitope peptides covering the amino acid sequence of the VP1 region of CV-A10 were screened by using the overlapping peptide method and verified by indirect enzyme linked immunosorbent assay(ELISA)and microneutral-ization inhibition assays.It has been found that candidate epitope P6(amino acid 39～53 in VP1 region of CV-A10)showed high reactivity with CV-A10 virus serum,and still showed neutralizing inhibition effect at 3.91 μg/mL dilution mass concentration,which was a potential neutralizing epitope.P6 antiserum was prepared by immunizing mice with P6 peptide,and microneutral-ization test was used to verify its protective effect.The results showed a 1:8.97 geometric mean neutralizing titer for P6 antise-rum,which identified P6 as the neutralizing epitope of CV-A10.In order to understand the conservativeness of P6 sequence in CV-A10,the amino acid sequence of the P6 was compared with the representative strains of CV-A10 genotype,and the results showed that P6 was highly conserved in CV-A10.It indicated that P6 is a conserved linear neutralization epitope of CV-A10,which has the potential to resist the infection of various genotypes of CV-A10,and can be used as a target for the development of CV-A10 epitope vaccine.This study aimed to screen and identify linearly neutralizing epitopes on the CV-A10 VP1 protein and to lay a foundation for the development of CV-A10 epitope vaccine and HFMD prevention.关键词
柯萨奇病毒A组10型/VP1蛋白/线性中和表位/疫苗/手足口病Key words
coxsackievirus A10/VP1 protein/linear neutral epitope/vaccine/hand,foot and mouth disease分类
医药卫生引用本文复制引用
甘燕媚,何韵怡,瞿颖,吴岳,刘启亮,刘洪波..柯萨奇病毒A组10型VP1蛋白线性中和表位的筛选和鉴定[J].激光生物学报,2024,33(2):160-166,7.基金项目
国家自然科学基金项目(81660280). （81660280）
