军事医学2024，Vol.48Issue(4)：260-266,7.DOI:10.7644/j.issn.1674-9960.2024.04.003
去泛素化酶Otulin基因心内膜敲除小鼠模型的构建及表型初步分析
Phenotypes of a mouse model of specific deubiquitinase Otulin gene knockout in the endocardium
摘要
Abstract
Objective To construct a mouse model with conditional knockout of deubiquitinase Otulin gene in the endocardium and to analyze the phenotype ofthe model.Methods A model ofmice with endocardium-specific conditional Otulin gene knockout was constructed with the Cre-loxP technique.The genotypes of Otulin gene knockout mice were determined by using polymerase chain reaction(PCR).Data on the reproduction of the knockout mice was statistically analyzed and the growth and development of the offspring were observed.The Cardiac function of mice was monitored by ultrasound and the changes of cellular components in blood were measured by a fully automatic hematology analyzer.Hematoxylin-eosin(HE)staining was used to analyze the pathological changes in all the tissues and organs ofthe offspring.Results An endocardium-specific Otulin gene knockout mouse model was established.The conditional knockout mice could grow and reproduce normally and the percentage of genotypes of their offspring conformed to Mende's law.Compared with littermate control mice,the conditional knockout mice had smaller body size,lighter weight,significantly enlarged spleens and spiking inflammatory cells in blood.In addition,the tricuspid valve defect and cardiac function were attenuated.Conclusion A mouse model of endocardium-specific Otulin gene knockout has been constructed.These mice show impaired cardiac function and obvious inflammatory response.关键词
线性泛素化/OTULIN/心内膜/条件性基因敲除/表型分析/小鼠Key words
linear ubiquitination/OTULIN/endocardium/conditional gene knockout/phenotypic analysis/mouse分类
医药卫生引用本文复制引用
吕亚迪,付业胜,张令强..去泛素化酶Otulin基因心内膜敲除小鼠模型的构建及表型初步分析[J].军事医学,2024,48(4):260-266,7.基金项目
国家重点研发计划(2021YFA1300200) （2021YFA1300200）
